患有急性传染性脑膜炎或脑炎的患者的脑液中的病原体概况:基于多重PCR和mNGS的多中心研究在中国,2018-2019年
Changcheng Wu1, Xinlei Sun1, Wei Zhang2
1National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
BMC infectious diseases
|November 25, 2025
概括
这项研究使用多重PCR和元基因组测序在中国确定了导致急性脑膜炎或脑炎 (AME) 的常见病原体. 结合这两种方法显著提高了病原体检测率,以更好地诊断和治疗AME.
科学领域:
- * 传染病 传染病
- * 神经科学是一门神经科学.
- * 微生物学 微生物学
背景情况:
- *急性脑膜炎或脑炎 (AME) 对健康构成重大风险,往往导致严重并发症或死亡.
- *在中国,对导致AME的病原体谱的系统研究是有限的.
- *了解病因对有效的诊断和治疗策略至关重要.
研究的目的:
- * 为了全面描述中国的AME病原体谱.
- * 评估多重PCR (MPCR) 和元基因下一代测序 (mNGS) 的诊断疗效.
- * 提供数据支持AME药物和疫苗的开发.
主要方法:
- *分析了来自中国 (2018-2019) 229名AME患者的脑脊髓液 (CSF) 样本.
- * 用43种病原体多重PCR (MPCR) 来进行病原体鉴定.
- * 甲基因组下一代测序 (mNGS) 应用于样本子集,以进行全面的病原体分析.
主要成果:
- *MPCR在31.88%的病例中检测到至少一个病原体.
- *mNGS证实了所有MPCR发现,并在以前未被诊断的病例中确定了额外的病原体.
- *常被检测到的病毒包括人类疹病毒 (HHV),腺病毒,肠病毒/鼻病毒 (Ev/Rv);细菌包括百日咳菌,流感菌和单细胞菌.
- *肠道病毒/鼻病毒 (Ev/Rv) 在青少年病例中普遍存在,传染性脑炎集中在中国东北部.
结论:
- *本研究提供了中国AME病原体的系统性表征.
- *MPCR和mNGS的组合显著提高了AME病原体的诊断率.
- *研究结果支持改善AME的诊断,治疗和对抗措施的开发.
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