在不同的温度变化和储存时间下对犬类红细胞透脆弱性的影响
Yi-Lun Tsai1,2, Davrald Webbe-Allen3, Wen-Shan Lee3
1Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan. yltsai@mail.npust.edu.tw.
BMC veterinary research
|November 25, 2025
概括
升高的温度 (25°C以上) 和延长的储存 (28天以上) 显著增加了狗全血中红细胞 (RBC) 的透脆弱性,可能会危及输血活力. 储存温度是影响红细胞膜完整性的一个比储存时间更大的因素.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 血液学 血液学 血液学
- 生物技术是生物技术.
背景情况:
- 红细胞透脆弱性 (EOF) 测试评估红细胞 (RBC) 膜完整性对输血质量.
- 最佳的血液袋储存温度通常为1-6°C,但温度波动可能导致血液溶解.
- 这项研究调查了时间和温度对狗全血质量的影响.
研究的目的:
- 为了确定储存时间和温度对犬全血质量对输血的影响.
- 通过使用EOF分析,在特定的储存间隔下在不同温度下分析透脆弱性的变化.
主要方法:
- 狗全血 (WB) 在CPDA-1中收集并存储.
- 血液样本在0,28和35天被分析.
- 在测试日,在EOF分析之前,样品在1,4,10,25和35°C下进行化24小时.
主要成果:
- 在所有测试日内,在25°C和35°C时观察到透性脆弱性增加.
- 存储超过28天并在35°C化的样本显示了透脆弱性的最显著差异.
- 平均体质脆弱性 (MCF) 不仅仅与温度有显著差异,而是受到温度和时间的结合的影响.
结论:
- 存储超过28天并暴露在25°C以上的温度下的狗全血可能会失去膜完整性和血液溶解.
- 温度上升显著影响了EOF,而储存时间的影响较小.
- 这些发现凸显了温度控制在维持存储的犬类红细胞生存能力方面的关键作用.
相关概念视频
Factors Influencing Microbial Growth: Temperature
1.1K
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
1.1K
Effects of Temperature on Free Energy
27.9K
The spontaneity of a process depends upon the temperature of the system. Phase transitions, for example, will proceed spontaneously in one direction or the other depending upon the temperature of the substance in question. Likewise, some chemical reactions can also exhibit temperature-dependent spontaneities. To illustrate this concept, the equation relating free energy change to the enthalpy and entropy changes for the process is considered:
27.9K
Factors Influencing Microbial Growth: Osmolarity
709
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
709
Factors Affecting Erythropoiesis
5.4K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
5.4K
Effect of Temperature Change on Reaction Rate
4.9K
The Arrhenius equation,
4.9K
Responses to Heat and Cold Stress
14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K


