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瘤免疫微环境和PD-L1测试的犬类泌尿细胞癌中的免疫表型
L V Muscatello1, G D'Annunzio2, T Franceschini3
1University of Bologna, Bologna, Italy.
BMC veterinary research
|November 25, 2025
概括
免疫检查点抑制 (ICI) 是一种有前途的癌症治疗方法. 这项研究特征了瘤免疫微环境 (TIME) 在犬类泌尿腺癌 (UCs),发现大多数UCs是免疫炎症和T细胞丰富的.
科学领域:
- 兽医瘤学 兽医瘤学
- 免疫学 免疫学 免疫学
- 癌症研究 癌症研究
背景情况:
- 免疫检查点抑制 (ICI) 提供了一种有前途的策略来对抗瘤免疫逃避.
- 对ICI的治疗反应受到瘤免疫微环境 (TIME) 的显著影响.
- 了解犬类泌尿腺癌 (UCs) 的时间对于开发有效的免疫疗法至关重要.
研究的目的:
- 在犬类UC中描述TIME.
- 为了研究TIME与编程死亡体1 (PD-L1) 表达之间的关系.
- 根据免疫细胞分布和PD-L1状态对UC进行分类.
主要方法:
- 追溯分析49只狗的UC.
- 针对PD-L1.1.的单抗体免疫组织化学.
- 对T细胞 (CD3),B细胞 (CD20) 和巨细胞 (IBA1) 的多重复合免疫组织化学.
- 计算机辅助图像分析 (QuPath) 用于量化免疫细胞和PD-L1表达.
- 瘤的分类为免疫炎症,免疫排除和免疫荒漠的表型.
主要成果:
- 在49个 (22%) 的UC中,有11个是PD-L1阳性.
- 大多数UC (40/49) 被归类为免疫炎症.
- 与PD-L1阴性瘤相比,PD-L1阳性瘤的巨细胞数量显著增加 (p=0.003).
- 与其他表型相比,免疫炎症瘤具有较高的T细胞密度和较低的巨细胞-T淋巴细胞比率 (分别为p=0.01和p=0.02).
结论:
- 狗的UCs主要是免疫炎症和T细胞丰富的.
- 一个UCs子集表达PD-L1并与增加的巨细胞透有关.
- 需要对T细胞和巨细胞两极分化进行进一步的研究,以改善犬类UCs的免疫反应分层.
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