在MCOF中双Fe活性位点工程 纳米酶用于协同和广谱抗菌疗法
Jingqi Liu1, Guimei Han1, Manying Li1
1Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P R China.
Small (Weinheim an der Bergstrasse, Germany)
|November 25, 2025
概括
一种具有双铁位点的新型金属共价有机框架 (MCOF) 显示出强大的抗菌活性. 这种新的纳米酶通过增强的反应性氧物种生成和静电相互作用,有效地对抗抗生素耐药细菌.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 纳米酶,特别是金属共价有机框架 (MCOFs),由于它们的过氧化酶 (POD) 类和氧化酶 (OXD) 类活动,显示出作为宽谱抗菌剂的前景.
- 传统的MCOF面临着不稳定的金属载荷结构和金属含量低的挑战,这限制了它们的有效性.
研究的目的:
- 开发一种新型的MCOF,具有增强的金属负载和双活性位点,以获得卓越的抗菌性能.
- 研究增强抗菌活性背后的机制,包括活性氧物种 (ROS) 生成和细菌膜相互作用.
主要方法:
- 采用一合成方法制造了一种新的MCOF,称为PA-FeFe COF,利用1,10-phenanthroline-5,6-dione (PA),TAPT和FeCl3.3.
- 合成的PA-FeFe COF以其结构性质,Fe载荷含量,POD类和光催化OXD类活动为特征.
- 对抗菌疗效被评估为对抗阳性和阴性细菌,并对抗生素耐药的细菌感染的伤口模型进行了体内实验.
主要成果:
- 该PA-FeFe COF实现了高Fe载荷,并显著增强了POD类和光催化OXD类活动.
- 超过99%的抑制率被观察到对格拉姆阳性和格拉姆阴性细菌,归因于协同ROS生成和强的正电荷促进细菌吸附.
- 该纳米酶表现出自我维持的H2O2生产,在五个周期内保持活性,并表现出快速的伤口愈合和高细菌清除 in vivo,具有良好的生物相容性.
结论:
- 开发的PA-FeFe COF代表了MCOF中金属装载的新基准,提供了高效的抗菌剂.
- 这项研究为设计多功能纳米酶以对抗抗生素耐药性感染提供了基本的见解.
- 该材料显示出在治疗细菌感染方面具有实践应用的巨大潜力.
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