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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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相关实验视频

Updated: Jan 10, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
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针对表观遗传修饰相关代谢物的有针对性的代谢策略,使用超高性能液体染色学-并联质谱法.

Wenbin Cai1, Weiyan Sun2, Ziru Wu1

  • 1Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China.

Journal of proteome research
|November 25, 2025
PubMed
概括

我们开发了一种敏感的方法来测量表观遗传代谢组,揭示了糖尿病心肌病中改变的关键代谢物. 这种方法有助于理解疾病中的基因表达调节.

关键词:
同酶共酶是一种共酶.糖尿病心肌病 糖尿病心肌病表观遗传学是指表观遗传学.代谢生物组的代谢生物组

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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相关实验视频

Last Updated: Jan 10, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics

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科学领域:

  • 代谢学 代谢学 代谢学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 生物化学 生化学

背景情况:

  • 表观遗传修饰调节了基因表达.
  • 与表观遗传学相关的代谢物,即"表观遗传代谢组",对于这些修改至关重要.
  • 了解表观遗传代谢组对于疾病研究至关重要.

研究的目的:

  • 开发一个全面的有针对性的代谢学方法来量化33个关键的表观遗传代谢物.
  • 建立一种敏感和可靠的分析方法来分析表观遗传代谢组.
  • 在糖尿病心肌病的小鼠模型中研究表观遗传代谢的变化.

主要方法:

  • 开发了一种使用液体染色学-质谱学的向代谢学方法.
  • 分析的代谢物包括乙-CoAs,NAD+代谢中间体和乙葡萄糖胺.
  • 经验证的方法性能具有高灵敏度 (LOQ < 16 ng) 和精度 (> 80%).

主要成果:

  • 成功地在糖尿病心肌病小鼠模型中描述了表观遗传代谢.
  • 确定了8种显著改变的代谢物.
  • 检测到与DNA/基因素甲基化,乙化和O-GlcNAcylation相关的变化.

结论:

  • 建立了一种可靠和敏感的方法来检测表观遗传代谢组变化.
  • 在与疾病相关的研究中证明了该方法的实用性,特别是糖尿病心肌病.
  • 提供了对影响疾病表观遗传修饰的代谢物变化的见解.