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Updated: May 12, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

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对Cas12a变体的PAM兼容性进行基准测试,用于高通量酵母的基因变体工程

Weiyu Xie1,2,3, Zhenkun Cai1,2,3, Zehua Bao1,2,3,4

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.

Applied and environmental microbiology
|November 25, 2025
PubMed
概括

我们开发了一个用于酵母基因组编辑的CRISPR/Cas12a系统,针对富含T的序列,以扩大基因变异工程超出CRISPR/Cas9的局限性.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas12a.这种植物是Saccharomyces cerevisiae.基因编辑 基因编辑

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Yeast As a Chassis for Developing Functional Assays to Study Human P53

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相关概念视频

Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...