植物中的病毒沉默抑制剂活动改变了虫的抗病毒免疫力和生育能力
Stephanie E Preising1, Jennifer R Wilson2, Glenn M Parker3
1Cornell University College of Agriculture and Life Sciences, Plant Pathology and Plant Microbe Biology, Ithaca, New York, United States; sep262@cornell.edu.
Phytopathology
|November 25, 2025
概括
马病毒操纵虫的繁殖和抗病毒防御. 抑制植物RNA干扰 (RNAi) 的病毒蛋白可以改变虫的生育能力和对昆虫病毒的易感性,揭示出复杂的共同进化动态.
科学领域:
- 植物病原体相互作用
- 昆虫病毒的共同进化
- RNA干扰 (RNAi) 机制是指RNA干扰的机制.
背景情况:
- 植物利用RNA干扰 (RNAi) 通过降解病毒RNA来对抗病毒感染.
- 植物病毒编码RNA沉默抑制剂 (VSRs) 来抵消宿主防御.
- 马叶卷病毒 (PLRV) P0蛋白通过其F-box动机准ARGONATE 1,抑制植物RNAi.
研究的目的:
- 研究PLRV P0蛋白在调节虫生育能力和抗病毒免疫力中的作用.
- 为了确定P0 F-box图案是否对于调节虫特征至关重要.
- 探索来自各种植物病毒的VSR对虫生物学的影响.
主要方法:
- 对PLRV P0蛋白的F盒突变体的生成和分析.
- 评估虫的生育能力和对Myzus persicae密度病毒 (MpDNV) 的敏感性.
- 从非虫传播的病毒中对虫生育能力和MpDNV标位的VSRs的评估.
主要成果:
- 要抑制虫的MpDNV抗病毒免疫力,需要一个功能性的P0 F-box动机,但不能调节虫生育能力.
- 其他植物病毒的沉默抑制剂也会影响虫的生育能力,但不会影响MpDNV水平.
- 虫在对植物RNAi路径变化的反应中表现出抗病毒免疫和生育能力的适应性调节.
结论:
- 植物病毒蛋白可以影响虫的生殖能力和免疫反应.
- PLRV P0的F-box动图差异性调节虫抗病毒免疫力和生育能力.
- 了解这些复杂的相互作用为管理载体传播的植物疾病提供了潜在的策略,通过准病毒蛋白来操纵昆虫载体.
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