相关实验视频
Updated: Jan 10, 2026

09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
15.0K
mRNA帽子积聚在压力颗粒中,对于它们的形成至关重要
Mingxin Guo1, Ying Feng1, Guantong Qi1
1State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010030, People's Republic of China.
Cellular and molecular life sciences : CMLS
|November 25, 2025
概括
压力颗粒通过mRNA盖的修改而形成,这对于细胞应激反应至关重要. 破坏RNA瓜-7-甲基转移酶 (RNMT) 阻止了压力颗粒的形成,突出显示了mRNA盖.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 压力颗粒 (SG) 是重要的细胞质器官,在细胞压力下形成.
- SGs防止mRNA翻译并保护RNA,但它们的形成机制尚不清楚.
- 在SG生物发生过程中mRNA帽子修饰的作用在很大程度上是未被探索的.
研究的目的:
- 为了研究mRNA帽子修饰在压力颗粒形成中的作用.
- 为了阐明mRNA被招募到压力颗粒中的机制.
主要方法:
- 使用系统级技术量化RNA帽表转录组.
- 在各种压力条件下分析压力颗粒中的mRNA盖组成.
- 基因敲除RNA关氨酸-7-甲基转移酶 (RNMT) 和化CTD相互作用因子1 (PCIF1).
- 蛋白质组,共免疫沉 (Co-IP) 和共聚焦免疫光分析.
主要成果:
- mRNA Cap1和非正规的帽子是显著丰富的压力颗粒.
- 根据不同的细胞应激条件,SG mRNA 盖的组成有所不同.
- RNMT和PCIF1敲击改变了mRNA盖的含量和组成.
- 在压力下抑制压力颗粒的形成.
- 通过与cap-binding蛋白的相互作用,mRNAs被招募到SG中.
结论:
- 在压力颗粒的形成中,mRNA盖的修改起着至关重要的作用.
- 帽子蛋白相互作用调解mRNA招募到压力颗粒.
- 这些发现揭示了mRNA上限在细胞应激反应中的重要性.
相关概念视频
Regulation of the Unfolded Protein Response
2.9K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.9K
Ribosomal RNA Synthesis
14.6K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.6K
Other Stress Responses in Bacteria
318
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
318
Nuclear Export of mRNA
8.6K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.6K
RNA Stability
35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Bacterial Protein Maturation
426
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
426

