玉米中核比率的候选基因通过在多个环境中结合GWAS和QTL映射来确定
Fengli Ye1,2, Ranjan K Shaw2, Babar Ijaz2
1School of Agriculture, Yunnan University, Kunming, 650500, China.
概括
这项研究确定Zm00001eb257890作为调节玉米核比率 (KR) 的关键基因. 这些发现为分子育种提供了目标,通过改进的同化分区来提高玉米产量.
科学领域:
- 植物遗传学 植物遗传学
- 玉米育种 玉米育种
- 分子生物学分子生物学
背景情况:
- 玉米核比 (KR) 对于玉米产量和同化分割至关重要.
- 了解KR的遗传基础对于作物改进至关重要.
研究的目的:
- 确定控制玉米核比率 (KR) 的基因和遗传位置.
- 为分子育种策略提供KR基因架构的见解.
主要方法:
- 开发了678个F9重组杂交系 (RILs) 的多父母种群 (MPP).
- 在三个环境中进行了表型评估.
- 使用全基因组复序 (WGRS),全基因组关联研究 (GWAS) 和QTL映射.
- 进行了qRT-PCR和基因结构分析,以验证候选基因.
主要成果:
- GWAS确定了99个KR的显著SNP,其中34个始终被检测到.
- QTL映射显示了35个QTL,其中包括两个稳定的QTL (qKR5-3,qKR5-7).
- Zm00001eb257890,编码一种氨酸/氨酸蛋白激酶,被确定为一个关键的候选基因.
- 在Zm00001eb257890中的G/A突变 (V1322I) 与低KR线的表达增加有关.
- Zm00001eb257890表达式显示了与KR值的显著负相关性.
结论:
- Zm00001eb257890是一种关键的候选基因,可以调节玉米核比率 (KR).
- 这项研究确定了Zm00001eb257890和KR之间的第一个关联.
- 这些发现为通过分子育种提高玉米产量提供了有价值的遗传标.
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