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Updated: Jun 27, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

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基于像素强度和基于对象的图像分析软件算法的比较,用于评估从嵌入氨酸的人类瘤样本中的部分的免疫组织化学染色

Sarah E Kimambo1, Josh Overton2, Nicole A Bouffard2

  • 1Department of Pathology and Laboratory Medicine, Larner College of Medicine, University of Vermont, Burlington, VT, 05405, USA.

Histochemistry and cell biology
|November 25, 2025
PubMed
概括

计算机辅助病理学软件显示了在癌症样本中分析免疫组织化学 (IHC) 标记物的潜力. 基于对象的分析比基于像素的方法提供了更一致的结果,尽管这两种方法都不能完全满足全面评估的需求.

关键词:
自动图像分析自动化图像分析在HALO中,我们使用HALO.这就是ImageScope的意象.基于对象的分析.病理学 病理学 病理学基于像素的分析.整个幻灯片成像 (WSI) 的成像.

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相关概念视频

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...