脱皮激活剂Edc3和Scd6与DHh1在转录后抑制饥饿诱导的途径中冗余作用
Rakesh Kumar1, Fan Zhang1, Shreyas Niphadkar2
1Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
eLife
|November 25, 2025
概括
酵母蛋白 Scd6 和 Edc3 冗余控制mRNA衰变和翻译,影响基因表达以响应营养的可用性. 它们的作用对于调节呼吸等细胞过程至关重要.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 酵母中的mRNA降解主要由Dcp1:Dcp2分解复合体介导.
- 解剖激活剂Edc3和Scd6以前被认为在mRNA衰变中起小作用.
研究的目的:
- 调查分离激活剂Edc3和Scd6在酵母mRNA衰变和转化抑制中的作用.
- 阐明Scd6,Edc3以及其他已知的切割因子 (如Dh1和Pat1) 之间的功能相互作用.
主要方法:
- 对缺乏Scd6和/或Edc3.3的酵母突变体进行RNA测序 (RNA-seq) 分析.
- 核糖体造型,以评估翻译抑制.
- 测量线粒体膜潜力和代谢中间体.
主要成果:
- Scd6和Edc3在针对许多mRNA进行降解方面表现出冗余功能,这种作用在单个突变者中被掩盖.
- 这些因素与Dh1和Pat1一起,共同调节mRNA脱落,衰变和转化抑制.
- 消除Scd6/Edc3会影响细胞代谢,模仿低葡萄糖的情况.
结论:
- Scd6 和 Edc3 与 Dhh1 具有冗余和并行作用,以控制 mRNA 分解和衰变.
- 这些蛋白质在调整基因表达以通过后转录调节来调整营养的可用性方面发挥着至关重要的作用.
- 这些发现揭示了一个复杂的调节网络,涉及Scd6,Edc3,Dhh1和Pat1在维持细胞平衡中.
相关概念视频
01:23Stringent Response in E. coli
272
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
272
02:04Co-activators and Co-repressors
8.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.4K
01:37The Unfolded Protein Response
6.2K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.2K
01:31Regulation of the Unfolded Protein Response
2.9K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.9K
01:32Export of Misfolded Proteins out of the ER
5.0K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
5.0K
00:50Anaphase Promoting Complex
3.3K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.3K