在垃圾填埋场的污水中对抗生素耐药性和病原体的基因组分析:环境影响
Ghassan Hadi Mohammed Jawad Diabil1, Amir Jalali1, Majid Komijani1
1Department of Biology, Faculty of Science, Arak University, Arak 38156-8-8349, Iran.
Journal of hazardous materials
|November 25, 2025
概括
垃圾填埋场的液含有抗生素耐药性基因 (ARG) 和细菌 (ARB),造成健康风险. 金属污染物影响ARG,表明这些污染物的生物修复潜力.
科学领域:
- 环境微生物学环境微生物学
- 环境化学环境化学
- 公共卫生 公共卫生
背景情况:
- 垃圾填埋场的液是抗生素,抗生素耐药细菌 (ARB) 和抗生素耐药基因 (ARG) 的重要来源.
- 这些污染物对人类,动物和环境健康构成重大风险.
- 了解液成分和ARG之间的相互作用对于风险评估和缓解至关重要.
研究的目的:
- 分析来自伊朗北部垃圾填埋场的液中的ARG的丰富性和多样性.
- 研究金属污染物,挥发性有机化合物 (BTEX) 和ARG配置文件之间的相关性.
- 探索液相关细菌对抗生素和ARG生物修复的潜力.
主要方法:
- 用于ARG分析的元基因组测序.
- 诱导合等离子体 (ICP) 分析用于金属量化.
- 气相色谱 (GC) 用于BTEX分析.
- 简短BRED和统计分析 (相关性,PERMANOVA) 来评估变量之间的关系.
主要成果:
- 在特定的液样本 (GL2,GL4) 中观察到更高的ARG丰度.
- 主要的细菌家族包括Pseudomonadaceae,Moraxellaceae和Enterobacteriaceae. 这些细菌家族是主要的.
- 金属污染物,特别是Sb和Zn,与总ARGs有很强的负相关性.
- 特定的金属与细菌家族有显著的相关性,这表明金属介导的抗性选择.
- 在不同地点,BTEX含量存在显著差异,和通常超过乙和.
结论:
- 垃圾填埋场的液是致病性ARB和ARG的储存库,受环境和微生物因素的影响.
- 金属污染物在塑造液中的ARG抗体中发挥着重要作用.
- 液相关细菌显示出生物化学治疗抗生素和ARG的潜力.
- 调查结果为有针对性的监测和风险管理提供了基础,以减轻ARG传播和保护公共健康.
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