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HIGD2通过改善线粒体功能,使大米具有低毒性应激耐受性

Geunmuk Im¹, Dongsu Choi²

¹Digital Breeding Convergence Division, National Institute of Agricultural Sciences, RDA, Jeonju, 54874, Republic of Korea.

Plant physiology and biochemistry : PPB

|November 25, 2025

概括

米基因OsHIGD2通过在缺氧期间维持线粒体功能来增强洪水耐受性. 过度表达改善了生存和呼吸,而淘汰突变体显示出减少了生长和细胞损伤.

科学领域:

植物生物学植物生物学
线粒体呼吸线粒体呼吸
压力生理学压力生理学

背景情况:

洪水和缺氧会损害米的线粒体能量代谢,阻碍生长.
了解低氧适应的分子机制对于作物弹性至关重要.

研究的目的:

研究大米缺氧诱导基因域蛋白2 (OsHIGD2) 在缺氧耐受性中的作用.
阐明 OsHIGD2 在低氧条件下维持线粒体呼吸的功能.

主要方法:

生成过基因大米系,过度表达OSHIGD2和CRISPR/Cas9淘汰突变物.
在低氧压力下评估的生存率,生长参数,电解质泄漏和细胞染色体c氧化酶活性.

主要成果:

OsHIGD2的表达是由低氧引起的.
过度表达线显示了增强的生存,生长,并保持了细胞染色体c氧化酶 (复杂IV) 活性.
淘汰突变者表现出增长迟缓和细胞损伤增加.

结论:

OsHIGD2显著提高了大米的低氧耐受性.
在氧气限制期间,OsHIGD2维持了线粒体功能和呼吸效率.
OsHIGD2 是农作物抗洪能力的关键调节者.

关键词:

细胞染色体c氧化酶的作用能量代谢能量代谢在HIGD2中使用HIGD2.氧气过低是因为缺氧.线粒体中的线粒体.大米大米大米大米大米潜水潜水是一种潜水.

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