N6-甲基氨酸表体转录重塑:主体RNA甲基化由PRRSV感染诱导的动态景观和功能分析
Meng Zhang1, Wei Wen1, Wenqiang Wang1
1Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
Veterinary microbiology
|November 25, 2025
概括
猪生殖和呼吸系统综合征病毒 (PRRSV) 感染改变了宿主细胞中的N6-甲基氨酸 (m6A) RNA修饰. 这种表观遗传调节影响病毒复制和宿主抗病毒防御,为PRRSV与宿主相互作用提供了新的见解.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- N6-甲基氨酸 (m6A) 是最常见的RNA修饰,对RNA代谢和功能至关重要.
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 对猪产业造成重大经济损害.
- 在PRRSV感染和宿主免疫反应中m6A的作用尚未完全理解.
研究的目的:
- 为了研究PRRSV感染期间m6A修饰的动态变化.
- 探索PRRSV与宿主相互作用中的m6A调节者的功能.
- 在PRRSV感染期间识别宿主基因和由m6A调节的途径.
主要方法:
- 用分子试验和m6A-seq来分析m6A的修饰.
- 评估了m6A调节者的表达和定位.
- 进行了对m6A调节器和基因的功能验证.
主要成果:
- 感染PRRSV诱导了宿主mRNA的显著变化m6A修改.
- 改变了m6A甲基转移酶,脱甲基酶和YTH域蛋白的表达和活性.
- 甲基化调节的宿主蛋白,包括指蛋白和转录因子,影响抗病毒防御.
- MAPK和IL-11信号通路被确定为m6A调节的目标.
结论:
- 在宿主细胞中,PRRSV感染会诱导一个动态的m6A景观.
- 一个修改在调节病毒复制和宿主抗病毒反应中起着至关重要的作用.
- 这些发现为复杂的病毒与宿主相互作用提供了新的见解,这种交互由m6A.
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