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动态瘤微环境从喉白血症改造为癌症,由单细胞转录组学揭示

Yunyi Liu¹, Peiyun Zhuang¹

¹Department of Voice, Zhongshan Hospital Xiamen University, Xiamen 361002, China.

|November 25, 2025

概括

这项研究揭示了喉白血病进展到癌症期间的关键细胞变化. 它确定了参与恶性转变的特定细胞类型和途径,为早期检测和治疗提供了新的点.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
基因组学就是基因组学.

背景情况:

喉白血病是一种常见的癌前病变,但其进展到癌症尚未完全理解.
了解细胞和分子变化对于早期检测和治疗策略至关重要.

研究的目的:

为了创建第一个单细胞图谱的喉白血病恶性进展.
为了识别关键的细胞亚群和推动这种转变的分子途径.
发现潜在的治疗点进行干预.

主要方法:

单细胞RNA测序 (scRNA-seq) 在十个临床标本上进行,包括白血病,早期癌症和对照组织.
计算分析被用来识别不同的细胞群和它们的相互作用.
途径分析的重点是确定关键的分子机制,包括氧化还原过程和细胞-细胞通信.

主要成果:

观察到瘤微环境的动态转变,涉及上皮细胞,纤维细胞和单核细胞.
确定了两个关键的上皮细胞亚群:Epi_4 (高度发育不良,具有高恶性潜力) 和Epi_5 (癌症,预后良好).
突出了恶性上皮细胞和相关的树皮细胞 (CAF,TAM) 之间的特定细胞-细胞通信通路 (JAG1-NOTCH4,CXCL5-CXCR1).
通过KEAP1-NRF2途径观察到与氧化还原相关基因的渐进激活.

结论:

这项研究提供了一个全面的单细胞表征喉白血病的恶性转变.
已识别的细胞亚群和通信通路为治疗策略提供了潜在的目标.
这些发现为改善喉癌的早期检测和机制研究奠定了基础.

关键词:

喉癌是一种喉癌.喉白血症 (Laryngeal Leukoplakia) 是一种严重的疾病.喉癌前病变的发生.一个单细胞RNA测序.

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