在无氧反应堆中,由定数灭驱动的酶活性和代谢途径调节,以增强甲生产
Zijing An1, Yibin Bao1, Yuwei Yang1
1Zhejiang Key Laboratory of Digital Intelligence Monitoring and Restoration of Watershed Environment, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Bioresource technology
|November 25, 2025
概括
使用Proteus sp.进行生物增量. 在无氧过程中,ZJ5显著提高了甲的产量. 这种增强与酶活性增加和微生物群落的有益转变有关,改善了整体治疗性能.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 厌氧消化 厌氧消化
背景情况:
- 无氧消化对于废物处理和生物气生产至关重要.
- 优化甲产量和工艺稳定性仍然是一个关键挑战.
- 生物增强为增强微生物过程提供了一个有希望的策略.
研究的目的:
- 为了评估Proteus sp.的影响. 在无氧消化过程中对甲产生的ZJ5生物增量.
- 调查潜在的机制,包括酶活性和微生物社区转移.
- 为了确定Proteus sp.的最佳剂量. ZJ5用于增强性能.
主要方法:
- 生物增强实验是使用不同剂量的Proteus sp.进行的. ZJ5 (0-280 毫克·L-1) 的情况.
- 测量了甲产量,酶活性 (蛋白酶,α-葡萄糖酶,乙酸酶,ETS) 和化学氧气需求 (COD) 移除.
- 修改后的Gompertz模型被用于动力分析.
- 进行了超基因组分析,以评估微生物社区结构和功能基因.
主要成果:
- 140 mg·L−1 Proteus sp. 的最佳剂量是 ZJ5导致甲产量增加了17%.
- 关键酶活动,特别是α-葡萄糖酶 (178%的增加) 显著增加.
- 甲基因组分析显示,性甲基生物 (Methanosarcina, Methanoculleus) 的丰富性增加,甲基生物发生路径丰富.
- 观察到改善了甲潜力,减少了滞后时间和增强了COD去除.
结论:
- 普罗蒂乌斯 (Proteus sp.) 的一种植物. 在无氧过程中,ZJ5有效地增强了甲回收.
- 细菌调节酶活性和微生物代谢途径,特别是甲基生成.
- 这项研究为通过有针对性的生物增量优化无氧消化提供了宝贵的见解.
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