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取决于tRNA的形状动态和折叠坐标的翻译调节是由全长的T-盒子 рибо开关
Xiaolin Niu1, Shanshan Cai2, Junzheng Wang1,3
1School of Life Sciences, Tsinghua University, Beijing, China.
Nature communications
|November 25, 2025
概括
T-盒子核糖开关通过使用tRNA氨基化状态来控制基因表达. 这项研究揭示了tRNA结合如何诱导构造变化,通过动态GAG链接器相互作用调节翻译启动或抑制.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- T-box рибо开关是调节基因表达的关键遗传元素.
- 它们的调节机制取决于感知转移RNA (tRNA) 的氨基化状态.
- 控制T-box рибо开关功能的动态形状变化仍然不完全理解.
研究的目的:
- 在单个分子水平上阐明T-box рибо开关调节的动态机制.
- 为了研究tRNA结合在T-盒子 рибо开关构造转换中的作用.
- 为了建模配转录折叠,tRNA结合和基因表达控制之间的相互作用.
主要方法:
- 单分子弗斯特尔共振能量转移 (smFRET) 研究.
- 研究了一种全长的翻译性ileS T-盒子核糖开关.
- 在连续的tRNA结合事件上分析了构造动态.
主要成果:
- T-box解码域独立折叠;初始tRNA结合促进了区分器域的折叠.
- 未充电的tRNA结合稳定了Antisequestrator (AntiS) 形状,促进了翻译启动.
- 充电的tRNA结合将T盒转移到Sequestrator形状,抑制翻译.
- 该GAG链接器,特别是G96,被确定为感知tRNA 3' termini的关键元素.
结论:
- T-盒子 рибо开关的调节涉及通过形态选择逐步的tRNA结合.
- GAG链接器在区分充电和未充电的tRNA中发挥着至关重要的作用.
- 综合转录折叠和动态转换的综合模型解释了T盒介导的基因调节.
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