工程酵母过氧体组装使得乙-CoA及其衍生的5-deoxyflavonoids的产量增加
Yongxing Li1, Bingjie Du1, Chun Li2,3,4,5
1Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China.
Nature communications
|November 25, 2025
概括
酵母中的工程过氧素 (PEXs) 增强了过氧体组合,将乙-CoA供应量提高了98%,从而改善了化学合成. 这种PEX策略可以跨酵母菌种进行扩展.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 乙-CoA对于生物合成至关重要,但在工程酵母细胞溶中通常是有限的.
- 参与脂肪酸氧化过程的过氧体可以被利用来供应乙-CoA.
研究的目的:
- 在S. cerevisiae中使用Peroxin (PEX) 工程来设计Peroxisome组件.
- 为了提高细胞质乙-CoA的可用性,以改善生物合成.
- 为了研究PEX-氧体组合-乙-CoA合成关系.
主要方法:
- 开发了一种素 (PEX) 工程策略,以调节S. cerevisiae中的氧体组合.
- 构建了强大的过氧体,增强了乙-CoA的生产.
- 从Y. lipolytica移植的PEX可以在S. cerevisiae中创建混合过氧体.
主要成果:
- 通过工程化百氧化体,通过工程化百氧化体实现了高达98%的乙-A供应改善.
- 升高了液性基因因标位,达到1102.4毫克/升.
- 证明了PEX介导的工程策略在不同酵母菌种中的适用性.
结论:
- 素工程有效调节氧体组合,并促进酵母中的乙-CoA合成.
- 这一策略增强了诸如liquiritigenin之类的有价值化合物的生产.
- 以PEX为媒介的方法为各种酵母菌种的代谢工程提供了一种多功能工具.
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