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使用RUBY报告员监测生菜中转基因表达的稳定性

Beth A Rowan^1,2,3, Megan Reeves^4,5, Claire Hays^4,5,6,7

¹The Genome Center, University of California, Davis, Davis, CA, USA. beth.rowan@oregonstate.edu.

Plant cell reports

|November 25, 2025

概括

lettuce 的 ubiquitin 促进剂和终止剂提供强大,稳定的跨代转基因表达,超过了 lettuce 中的花草马赛克病毒 35S 促进剂. 这一发现为开发稳定的转基因生菜提供了有价值的工具.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

在生菜中稳定转基因表达仍然是一个挑战,像花马赛克病毒35S这样的常见促进者表现出不稳定性.
关于在生菜中稳定转基因表达的各种促进剂/终结剂组合的研究有限.

研究的目的:

评估和比较不同转基因表达结构在生菜中的有效性.
确定强大的促进子和终结子组合,以在生菜中实现稳定和强大的转基因表达.

主要方法:

利用非侵入性的RUBY报告员系统来监测转基因表达.
在两种菜品种中评估了三种不同的表达结构 (LsUBI,AtUBI/tRBCS,35S/tHSP).
在再生期间跟踪转基因表达,初级转基因发育和两个后续代.

主要成果:

在LsUBI的促进者/终结者组合中,在所有阶段和世代中表现出强烈,均和稳定的表达.
在AtUBI促进剂/tRBCS组合中,表达程度略低,中间静音.
35S促进剂/tHSP组合最初表现出强烈的表达,但频繁沉默.

结论:

LsUBI促进器/终结器对生菜中强烈,均和稳定的转基因表达非常有效,最大限度地减少了沉默.
在AtUBI促进剂/tRBCS组合提供了一个可行的替代品,稳定的转基因表达在,特别是当中间表达水平是理想的.

关键词:

工厂转化工厂转化工厂转化工厂转化活动主办人活动主办人红宝石 (Ruby) 是一种红宝石.转基因沉默是对转基因进行沉默的.

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