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通过使用DUET生成的虚拟PAS染色改善冷脏活检的解释

Kuang-Yu Jen, Willy Ju, Srishti Seth

medRxiv : the preprint server for health sciences

|November 26, 2025

概括

虚拟周期性酸-Schiff (vPAS) 染色改善病理学家在从冷活检中评估捐赠的活力时的一致性. 这种方法增强了间歇性纤维化/管管缩和炎症的评估,有助于移植决策.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
病理学病理学病理学
医疗成像医学成像

背景情况:

对于冷的捐赠脏活检,标准的血素和欧 (H&E) 染色面临着冷文物和有限的病理学家专业知识等挑战.
目前的方法缺乏快速,经济高效的方法来产生虚拟的特殊污点,以量化结部分的组织病理特征.

研究的目的:

评估DUET生成的虚拟周期性酸-Schiff (vPAS) 染色的实用性,以评估使用冷活检的捐赠的活力.
为了比较病理学家的诊断一致性,仅使用H&E与H&E与vPAS染料相结合.

主要方法:

DUET软件通过将H&E染色的冷脏活检中的亮场和光图像结合起来,生成vPAS染色.
脏和一般病理学家在29次脏活检中评估了间歇性纤维化/管管缩 (IF/TA),炎症和动脉硬化.
评估仅在H&E图像和H&E与vPAS图像之间进行了比较.

主要成果:

在使用vPAS染色时,病理学家的一致性 (ICC分数) 改善了IF/TA百分比,炎症百分比和动脉硬化数.
病理学家还显示,硬化性球体百分比的一致性有所改善.
对于动脉动脉质的评估没有观察到任何改善.

结论:

与单独的H&E相比,冷脏活检上的虚拟PAS染色提高了关键可行性指标的病理学家间一致性.
这种技术有可能减少诊断变异性,改善捐赠的利用率和移植决策.

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