SLC11A2 影响肠道上皮质中的营养免疫力
Emilia S Norberg1, Trina L Westerman2, Eddy Cruz2
1Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, VT.
bioRxiv : the preprint server for biology
|November 26, 2025
概括
肠道上皮层使用SLC11A2转运器来限制铁和等必需金属,从而限制感染期间的病原体生长. 这突出了对肠道细菌的关键宿主防御机制.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 病原体与宿主相互作用涉及对必要的微量金属进行竞争.
- 营养免疫是一种宿主防御策略,用于限制金属对病原体的可用性.
- 肠上皮细胞 (IECs) 在金属吸收中发挥作用,是肠道病原体的目标.
研究的目的:
- 研究IEC内在因素在对肠道病原体的营养免疫力中的作用.
- 在肠道中感染沙门氏菌时,绘制金属竞争的时空动态图.
- 为了确定金属载体SLC11A2在IEC中对主机防御的贡献.
主要方法:
- 在牛的结合性肠循环模型中使用了Salmonella enterica Typhimurium (STm) 血清菌与金属响应的光报告器.
- 在IEC中使用SLC11A2的基因淘汰模型.
- 使用基于光的生物传感器和细菌基因删除突变体来确定特定的金属限制.
主要成果:
- 在感染初期,STm在肠道粘膜中经历了铁,和的时间和细胞特异性限制.
- 在IEC中SLC11A2的淘汰导致STm复制的增强,这表明载体的保护作用.
- 和铁的限制被确定为SLC11A2限制细菌增殖的机制.
结论:
- 通过SLC11A2介导的金属封存是肠表皮的内在防御机制.
- 这种上皮的防御限制了肠道细菌 (如沙门氏菌) 的增殖.
- 了解这些机制可以为应对胃肠道感染的策略提供信息.
关键词:
生物科学 生物科学微生物学 微生物学沙门氏菌 (Salmonella enterica) 已经被发现.肠道疾病是一种肠道疾病.胃肠道的胃肠道是什么样子的肠道上皮细胞是肠道上皮细胞金属的平衡是金属的平衡.更多相关视频
06:43Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
Published on: May 26, 2021
6.3K
09:24Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
Published on: February 11, 2021
7.8K
相关概念视频
Renewal of Intestinal Stem Cells
3.1K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Transcytosis of IgG
4.0K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.0K
Glucose Transporters
27.1K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
27.1K
