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Updated: Jan 10, 2026

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定义和检测昼夜基因表达中的全球转录幅度

Michael Saint-Antoine¹, Ron C Anafi¹

¹Department of Medicine, Chronobiology and Sleep Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, US.

bioRxiv : the preprint server for biology

|November 26, 2025

概括

个人在他们的转录组中表达基因表达节奏的幅度上表现出一致的差异. 在皮肤,脂肪,肌肉和血液组织中观察到这种全球节律幅度模式,这表明人类昼夜生物学的基本方面.

科学领域:

时间生物学时间生物学
基因组学就是基因组学.
人体生理学人体生理学

背景情况:

循环节律调节基因表达,观察到振荡幅度的个体变化.
之前的研究集中在基因特异幅度或核心时钟基因突变的影响.
对于跨个体的昼夜振幅的全球性,全转录组范围的模式的存在是未知的.

研究的目的:

为了确定转录幅度的差异是基因特异性的还是反映了整个转录组的全球模式.
调查人体皮肤和其他组织中对特定主体的全球幅度趋势.
评估核心时钟基因表达和全球振幅模式之间的相关性.

主要方法:

用Cosinor回归重新分析了四个人类皮肤时间序列的转录数据集.
应用非参数的,基于变换的统计测试来评估全球幅度的显著性.
从脂肪,肌肉和血液组织中分析了6个额外的人类时间过程数据集.

主要成果:

基因幅度按个体分组,表明个体特定的全球幅度趋势.
这种全球幅度趋势在多个组织中的许多受试者中具有统计学意义.
皮肤表皮的特定对象的全球振幅预测了皮肤中的振幅,以及在不同的高峰时间.
全球幅度趋势在脂肪,肌肉和血液数据集中复制.
核心时钟基因组织和振幅与特定主体的全球振幅没有很强的相关性.

结论:

个人表现出一致的,主体特定的全球模式,在他们的昼夜转录节律的幅度.
这种全球振幅现象是独立于组织的,并且存在于各种人体组织中.
主体特定的全球振幅与核心时钟基因表达指标没有很强的联系.

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