转录因子-促进剂相互作用用于PSY/PSYR介导的根生长调节
Jin C-Y Liao1, Anne-Maarit Bågman2,3,4, Angie J Liu1
1Department of Plant Pathology, University of California, Davis, CA, USA.
bioRxiv : the preprint server for biology
|November 26, 2025
概括
植物生长依赖于精确的基因调节. 研究人员绘制了Arabidopsis中转录因子相互作用的地图,确定了CYTOKININ RESPONSE FACTOR 10 (CRF10) 作为影响根部发育的关键生长抑制剂.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学和基因组学 在
- 植物生理学 植物生理学
背景情况:
- 精确的基因表达的控制对植物生长至关重要.
- 含有硫酸铁 (PSY) 和它们的受体 (PSYRs) 的植物路调节了Arabidopsis thaliana的生长压力权衡.
- 对于PSY/PSYR基因的转录调节还没有得到充分的了解.
研究的目的:
- 在Arabidopsis thaliana中的PSY/PSYR通路内绘制转录因子 (TF) -促进体相互作用.
- 确定影响根生长的关键调节者.
- 调查细胞基因反应因子10 (CRF10) 在根部发育中的作用及其对3A拓糖酶 (TOP3A) 的潜在影响.
主要方法:
- 高通量增强的酵母单杂交 (eY1H) 选与DNA亲和净化测序 (DAP-seq) 数据相结合.
- 对25个TF突变的功能分析.
- 在CRISPR/Cas9中介的促进器编辑.
主要成果:
- 确定了1,207个TF-促进器相互作用,定义了PSY/PSYR途径的全球监管网络.
- 12个TF突变物显著影响了根生长,大多数作为抑制剂.
- 细胞基因反应因子10 (CRF10) 被确定为一种强大的生长抑制剂,其结合动机在PSYR3促进体中也位于TOP3A外子中.
- 通过CRISPR/Cas9编辑CRF10结合基因或周围区域,增强了根生长.
结论:
- 这项研究阐明了TF-促进器相互作用网络,该网络控制了Arabidopsis中的PSY/PSYR通路.
- CRF10作为根生长的显著抑制剂,可能通过其与PSYR3促进体和TOP3A外基子的相互作用.
- 通过基因组编辑破坏CRF10结合动机或相关的TOP3A外因子区域可以增强根生长.
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