深紫外激光扫描显微镜与集成的1050nm光谱域光学连贯性断层扫描用于多对比组织成像
Matthew T Martell1, Nathaniel J M Haven1, Mohammad Hadi Masoumi1
1Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.
Biomedical optics express
|November 26, 2025
概括
这项研究引入了一种新的光学成像系统,用于快速,无幻灯片的厚组织组织学分析. 该系统结合了深度紫外线显微镜和光学连贯性断层扫描,以提高外科手术边缘评估.
科学领域:
- 生物医学光学 生物医学光学
- 医疗成像医学成像
- 组织病理学 组织病理学
背景情况:
- 无幻灯片显微镜对于手术边缘分析至关重要.
- 最少处理的厚组织需要先进的成像技术.
- 目前的方法在复杂样本的速度和分辨率方面存在局限性.
研究的目的:
- 开发和演示用于组织学类组织可视化的多式光学成像系统.
- 为了使厚组织样本的快速,无幻灯片分析.
- 通过先进的成像来改善手术边缘评估.
主要方法:
- 一个新的系统结合了266nm激发光,266nm共聚焦反射和1050nm光谱域光学连贯断层扫描 (OCT).
- 使用深紫外线共焦反射和DAPI光用于表面成像.
- 采用深度学习进行虚拟血素和 (H&E) 染色.
- 集成的横截面OCT用于地下形态可视化.
- 开发了一种定制的反射镜头,用于同时进行高分辨率紫外线和OCT成像.
主要成果:
- 通过使用深紫外线反射和光,在组织表面实现了类似组织学成像.
- 通过深度学习算法展示了虚拟的H&E染色.
- 展示了近红外OCT的地下组织形态可视化.
- 成功成像了 ex vivo 小鼠脏,肝脏和人类乳腺组织.
- 经过验证的同时高分辨率,无偏差成像能力.
结论:
- 开发的多式光学成像系统为无幻灯片组织学分析提供了强大的工具.
- 这项技术在改善外科手术边缘评估方面具有重大潜力.
- 深紫外线显微镜和OCT的结合提供了全面的组织可视化.
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