相关实验视频
Updated: Jan 10, 2026

04:08
Mouse Model of Surgical Uterine Injury and Subsequent Pregnancy Outcomes
Published on: June 27, 2025
1.0K
子宫内膜损伤及其由介质干细胞的救援取决于雌激素周期阶段
Ramanaiah Mamillapalli1, Ying Liu1, Yuping Zhou1
1Department of Obstetrics, Gynecology & Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, USA.
Journal of cellular and molecular medicine
|November 26, 2025
概括
在期期间由子宫内膜损伤引起的阿舍尔曼综合征 (AS) 不孕症可以通过骨髓衍生中酶干细胞 (BM-MSCs) 恢复. 治疗主要通过免疫调节起作用,而不是细胞移植,恢复子宫功能.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞疗法是一种干细胞疗法.
- 妇科 妇科医生 妇科
背景情况:
- 阿舍尔曼综合征 (AS) 涉及子宫痕和不孕不育,通常是在手术切除后,手术治疗的成功有限.
- 骨髓衍生的介质干细胞 (MSCs) 显示出恢复AS的子宫功能具有前景.
- 雌性周期阶段对子宫内膜损伤和MSC治疗结果的影响尚不清楚.
研究的目的:
- 在小鼠模型中调查雌激期与雌激期期间子宫内膜损伤对生育能力的差异影响.
- 评估骨髓 (BM) 衍生MSC治疗在子宫内膜损伤后恢复生育能力的疗效.
- 阐明BM-MSC在子宫内膜修复中的作用机制,重点关注移植与免疫调节.
主要方法:
- 在小鼠的特定雌激周期阶段 (雌激或雌激) 诱导子宫内膜损伤.
- 评估受伤和/或BM-MSC治疗后的生育结果.
- 免疫光分析以检测BM-MSC移植 (维门丁,细胞克拉).
- 通过qRT-PCR分析免疫细胞透 (CD45+) 和基因表达 (Ccl3,Il-1β,Mmp3).
主要成果:
- 雌激期内宫内膜损伤不会影响生育能力,而雌激期内宫内膜损伤会导致不孕.
- 在消化期受伤后的BM-MSC治疗恢复了生育能力.
- BM-MSC治疗没有导致显著的移植,但在子宫中增加了CD45+免疫细胞.
- 基因表达分析显示,受伤+BM-MSC组的Ccl3,Il-1β和Mmp3增加,这表明免疫调节机制存在.
结论:
- 腹产期期间子宫内膜损伤是AS相关不孕症的关键因素.
- BM-MSC疗法有效地恢复了经过腹期子宫内膜损伤后的生育能力.
- 在这个模型中,BM-MSCs的治疗效果主要通过免疫调节而不是直接的细胞移植.
相关概念视频
Menses Phase
970
The uterine cycle begins with the menstrual phase, which is considered day one of the cycle and typically lasts about five days. This phase is characterized by the degeneration and shedding of the stratum functionalis, the functional layer of the endometrium.
When fertilization does not occur, the corpus luteum deteriorates, causing a significant drop in the levels of estrogen and progesterone in the body. This hormonal decrease triggers the release of prostaglandins, which cause the uterine...
When fertilization does not occur, the corpus luteum deteriorates, causing a significant drop in the levels of estrogen and progesterone in the body. This hormonal decrease triggers the release of prostaglandins, which cause the uterine...
970
Mesenchymal Stem Cells
5.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.5K
Proliferative Phase
1.2K
The proliferative phase typically occurs after menstruation and lasts between 6 to 13 days in a standard 28-day cycle. This phase involves the reconstruction of the endometrium, guided by estrogen produced by the developing ovarian follicle.
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
1.2K
The Menstrual Cycle
3.0K
The menstrual cycle is a recurrent sequence of changes in the uterine endometrium, specifically its functional layer, the stratum functionalis. This cycle prepares the uterus for potential pregnancy. This cycle typically spans 21–35 days, averaging 28 days, and aligns with the ovarian cycle, regulated by fluctuating levels of ovarian hormones, primarily estrogen and progesterone.
The menstrual phase occurs from days 1 to 5 and involves the shedding of the stratum functionalis, as a...
The menstrual phase occurs from days 1 to 5 and involves the shedding of the stratum functionalis, as a...
3.0K
Ovarian Cycle
3.3K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
3.3K
Hormonal Regulation of the Menstrual Cycle
1.4K
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
1.4K

