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一个用于细胞分离的生物传感平台:模拟一种利用电介质指纹的电介质微流体装置

Reza Hadjiaghaie Vafaie¹, Elnaz Poorreza¹, Sobhan Sheykhivand²

¹Department of Electrical Engineering, University of Bonab, Bonab 5551761167, Iran.

Biomimetics (Basel, Switzerland)

|November 26, 2025

概括

这项研究引入了一种新型的二电电泳 (DEP) 系统,用于从血液中分离循环瘤细胞 (CTC). 生物体设备在将癌细胞与白细胞 (WBCs) 分离方面实现了高效率,有助于早期发现癌症.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
在瘤学瘤学.

背景情况:

循环瘤细胞 (CTC) 是癌症诊断和治疗监测的关键生物标志物.
有效地将CTC从血液中分离出来,对于及时进行瘤干预至关重要.
介电泳 (DEP) 是基于介电特性进行细胞分离的有希望的无标签技术.

研究的目的:

开发和验证一种基于DEP的新型生物系统,用于从白细胞 (WBC) 中分离MDA-MB-231乳腺癌细胞.
为了实现癌细胞的连续,无标签的分离,具有高效率和保存细胞活力.
调查影响DEP微流体装置分离效率的因素.

主要方法:

一个多阶段DEP微流体装置的设计,使用独特的细胞介电指纹进行分离.
实施三个不同的分离阶段来分离特定的细胞类型 (MDA-MB-231,B-淋巴细胞,单细胞,粒细胞).
电潜,速度场,压力分布,细胞DEP力和粒子路径的计算分析.

主要成果:

对于癌细胞,DEP系统的预测分离效率约为92%.
该设备成功地分化和分离了包括癌细胞在内的各种血液细胞组成部分.
分析确定了影响分离效率的电极电位和通道宽度等关键参数.

结论:

开发的DEP生物系统为CTCs的无标签隔离提供了一种可行和高效的方法.
这项技术有可能改善早期癌症检测和个性化治疗策略.
对优化操作参数的进一步调查可以提高系统在临床应用中的性能.

关键词:

生物医学生物医学仿生生物技术 (BIONIC) 是一种癌症癌症癌症癌症癌症循环中的瘤细胞.导电泳分离分离分离.微流体学在微流体学方面

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