Jove
Visualize
联系我们

相关概念视频

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

De Novo Engineered Living Materials via Elastin-Like Polypeptide-Mediated Self-Assembly.

ACS synthetic biology·2026
Same author

Modeling of Conjugative- and Phage- Mediated CRISPR-based System against Antimicrobial Resistant Bacteria.

IEEE transactions on bio-medical engineering·2026
Same author

<i>Bifidobacterium</i><i>animalis</i> subsp. <i>lactis</i> BB-12 Primes Epithelial Antiviral Defenses and Restricts Influenza A Virus Replication in Human Intestinal Organoid-Derived Monolayers.

Microorganisms·2026
Same author

Enteric botulinum neurotoxins facilitate infection by <i>Salmonella</i> and <i>Shigella</i>.

Science advances·2026
Same author

Exploring the Impact of DNA Methylation on Gene Expression in CRC: A Computational Approach for Identifying Epigenetically Regulated Genes in Multi-Omic Datasets.

Cancers·2026
Same author

Audio-guided exercise at home: Preliminary findings from a pilot study on physical activity for people with low vision.

PM & R : the journal of injury, function, and rehabilitation·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 15, 2026

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

13.3K

使用工程细菌生物传感器检测胆汁酸转化微生物的查试验.

Debora Dallera1,2, Daniele Pastorelli1,2, Massimo Bellato3,4

  • 1Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Via Ferrata 5, 27100 Pavia, Italy.

Biosensors
|November 26, 2025
PubMed
概括

一种新的细菌生物传感器测试幕检测了微生物中的胆盐酸酶 (BSH) 活性. 这种可访问的方法可以识别产生BSH的菌株,这对于了解胆酸代谢和相关疾病至关重要.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.这就是TcpH.TcpPP 的意思是细菌传感器是一种细菌传感器.胆汁盐 胆汁盐 胆汁盐校准曲线上的校准曲线.度测定法测定度测试方法乳酸细菌是一种乳酸细菌.特殊性的特异性合成生物学 合成生物学

更多相关视频

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
08:42

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport

Published on: November 27, 2016

11.6K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.4K

相关实验视频

Last Updated: Jun 15, 2026

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

13.3K
Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
08:42

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport

Published on: November 27, 2016

11.6K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.4K

科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 酶学 是一种酶学.

背景情况:

  • 胆盐化酶 (BSH) 是肠道微生物胆酸 (BA) 代谢的关键.
  • BA池的失调与各种人类和动物疾病有关,需要确定BSH活动.
  • 现有的BSH查方法往往是昂贵的,不敏感的或复杂的.

研究的目的:

  • 开发一种用于查微生物BSH活动的新,可访问和敏感的测试方法.
  • 使用细菌生物传感器通过光来报告BA度以检测BSH.
  • 通过在受控范围内测试初级BA并解决矩阵效应来确保测试特异性.

主要方法:

  • 开发一种用于检测胆酸 (BAs) 的细菌生物传感器.
  • 使用生物传感器对微生物BSH活动进行选试验.
  • 对复合大肠杆菌菌株*表达来自*Lactobacillus johnsonii* PF01和*Lactobacillus rhamnosus* GG的BSH基因的测定方法的评估.
  • 评估微生物生长介质对生物传感器输出的矩阵效应.

主要成果:

  • 该试验成功检测了在测试的微生物菌株中BSH活性.
  • 结果与之前对评估的酶的BSH活性测定一致.
  • 该试验在解决生长介质的矩阵效应方面表现出强度.

结论:

  • 开发的生物传感器测定方法提供了一种可复制和可访问的方法,用于查培养微生物中的BSH活性.
  • 这种测试支持识别BSH生产菌株的健康和农业行业相关.
  • 该测试与自动化协议兼容,提高了其实用性.