基于对IgG抗体Fc段的亲和DNA功能性干构建一个免疫传感器
Qianyu Yang1, Zhiwei Liu1, Xinrui Xu1
1State Key Laboratory of Chemistry for NBC Hazards Protection, Beijing 102205, China.
Biosensors
|November 26, 2025
概括
研究人员开发了一种基于DNA的新型配体 (A-DNAFL) 用于抗体固定,为蛋白A提供了具有成本效益和高度可修改的替代品. 这种新工具可以提高生物传感器的性能,用于诸如素检测等应用.
科学领域:
- 生物技术是生物技术.
- 生物感应是一种生物感应.
- 分子相互作用 分子相互作用
背景情况:
- 像蛋白A这样的Fc片段结合蛋白广泛用于抗体净化和固定.
- 现有的方法面临成本,可修改性和特定结合效率的局限性.
研究的目的:
- 设计和验证一种新型亲和性DNA功能联结体 (A-DNAFL),作为抗体不动化的蛋白A的替代品.
- 优化A-DNAFL结合条件并描述其与小鼠IgG抗体的相互作用.
- 构建和评估一种用于检测辛的免疫传感器,使用A-DNAFL进行抗体固定.
主要方法:
- 对A-DNAFL结合亲和力 (KD = 6.59 × 10-8) 对小鼠IgG的查和实验验证.
- 在不同的pH值和离子强度条件下对结合效率的系统评估.
- 竞争性结合测定,分子对接模拟和免疫传感器制造用于酸盐检测.
主要成果:
- 甲-DNAFL的结合性能与蛋白A的结合性能相当,在pH值7.2-7.4.4的PBST-B缓冲物中具有最佳的固定.
- 分子对接揭示了关键相互作用 (结合,疏水),稳定了A-DNAFL-Fc片段复合体.
- 开发的瑞免疫传感器实现了低检测极限 (30.5 ng/mL),比蛋白质A方法提高了64倍,具有良好的重复使用性.
结论:
- A-DNAFL是一种有前途的新型材料,用于抗体导向固定,作为蛋白A的可行替代品.
- 它的高可修饰性,低成本和小分子量为生物传感和抗体净化提供了广泛的潜力.
- 开发的免疫传感器展示了A-DNAFL在敏感和特定分析物检测中的实际实用性.
相关概念视频
Enzyme-Linked Immunosorbent Assay
17.2K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
17.2K
Immunocytochemistry and Immunohistochemistry
13.4K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...
13.4K
Antibody Structure
65.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.2K
Immunogold Electron Microscopy
5.3K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.3K
Immunofluorescence Microscopy
12.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
12.9K
Immunoglobulin-like Cell Adhesion Molecules
4.1K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.1K


