Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Centrosome Duplication02:25

Centrosome Duplication

4.8K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.8K
Meiosis II02:02

Meiosis II

48.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
48.9K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.3K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.3K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

5.2K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.2K
Cohesins02:20

Cohesins

5.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.4K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

69.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
69.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Assessing Surface Binding Events on Golgi Microsomes Isolated from Cancer Cells by Capillary Electrophoresis.

Analytical chemistry·2026
Same author

Stearoyl-CoA Desaturase-1 Drives Tumor Growth by Interacting With Histone Deacetylase-2 and Deacetylating Nucleophosmin-1.

MedComm·2026
Same author

Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.

Nature communications·2026
Same author

Beyond the stop: Oxadiazole TRIDs restore LRBA protein expression in nonsense-driven primary immunodeficiency.

Molecular therapy. Nucleic acids·2026
Same author

ADAMTS2: More than a procollagen N-proteinase.

Genes & diseases·2025
Same author

The ADAMTS2 metalloproteinase inhibits tumor growth by regulating the innate immune system.

Cancer cell international·2025

相关实验视频

Updated: Jan 10, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K

减少RhoGDI2表达破坏了中枢细胞功能,并促进了线粒错误.

Mudrika Tripathi1, Nancy Garbacki1, Jérôme Willems1

  • 1Laboratory of Connective Tissues Biology, GIGA-Cancer, University of Liège, 4000 Liège, Belgium.

Cells
|November 26, 2025
PubMed
概括
此摘要是机器生成的。

在癌症中,RhoGDI2 (Rho Guanine 分解抑制剂2) 调节细胞增殖和中心体功能. 沉默RhoGDI2会影响NK细胞杀死癌细胞,揭示了它在癌症进展和免疫方面的双重作用.

关键词:
对于rhoGTPases来说,这是一个很好的方法.中心体 中心体 中心体 中心体染色体凝聚是一种染色体的凝聚.免疫突触的突触发生.主要的乳毛是主要的.

更多相关视频

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
07:06

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

Published on: June 23, 2023

4.2K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.7K

相关实验视频

Last Updated: Jan 10, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K
Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
07:06

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

Published on: June 23, 2023

4.2K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.7K

科学领域:

  • 细胞生物学 细胞生物学
  • 癌症生物学 癌症生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • RhoGDI2是一种Rho GTPase调节剂,参与细胞骨组织和细胞存活.
  • 它的表达在人类癌症中有所不同,与预后相关.
  • 在免疫细胞中RhoGDI2的表达很高,主要的乳毛和免疫突触之间有相似之处.

研究的目的:

  • 研究RhoGDI2在癌症中的双重作用背后的机制.
  • 探索RhoGDI2在癌细胞增殖,中心细胞调节和免疫细胞活性中的功能.

主要方法:

  • 在人类癌症细胞系中抑制RhoGDI2表达.
  • 评估增殖率,中心体数量和纤毛生成.
  • 沉默RhoA以调查GTPase参与的情况.
  • 在NK细胞中抑制RhoGDI2,以评估免疫突触功能.

主要成果:

  • 抑制RhoGDI2降低了癌细胞的扩散,诱导了超数的中心体,并抑制了纤维生成.
  • 通过抑制RhoGDI2引起的RhoA沉默部分挽救的中心细胞和状细胞缺陷.
  • 在NK细胞中抑制RhoGDI2显著损害了它们通过免疫突触杀死癌细胞的活性.

结论:

  • 在人类癌症中,RhoGDI2在中心细胞功能中发挥着新的作用.
  • RhoGDI2对于免疫细胞中的免疫突触功能至关重要,影响抗癌活性.
  • 这些发现解释了观察到的RhoGDI2在癌症进展和免疫方面的双重作用.