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在使用MonoTrap微型提取的整体和腺体唾液中对挥发性有机化合物的全面GC/MS分析

Asuka Mori¹, Masae Kuboniwa², Eiichiro Fukusaki^1,3

¹Department of Biotechnology, Graduate School of Engineering, The University of Osaka, Osaka 565-0871, Japan.

|November 26, 2025

概括

唾液挥发性有机化合物 (VOC) 可以作为非侵入性生物标志物. 这项研究开发了一种方法来分析小量的唾液中的VOC,识别与腺体唾液相比,整个唾液中不同的微生物影响的化合物.

科学领域:

生物化学生物化学
分析化学分析化学
微生物学微生物学

背景情况:

唾液挥发性有机化合物 (VOC) 正在成为用于疾病检测的非侵入性生物标志物.
与整个唾液相比,腺体唾液提供了更纯净的生物流体,而整个唾液是一种复杂的混合物.
了解VOC差异对于确定可靠的生物标志物至关重要.

研究的目的:

建立一种可靠的方法,从小量的唾液 (100微升) 中分析VOC.
为了识别可区分腺体和全唾液的VOCs.
调查整个唾液中明显的VOC配置文件的来源.

主要方法:

开发了一个使用二甲进行唾液VOC分析的MonoTrap提取协议.
实施了两步空白分析以消除采样设备的工件.
使用OPLS-DA和Wilcoxon签名等级测试进行VOC数据的统计分析.

主要成果:

确定了72种挥发性有机物,通过空白分析排除了15种人工物.
发现了10个关键的VOC,与腺体唾液相比,在全唾液中显著增加.
发现整个唾液中升高的VOCs主要是微生物代谢产物,表明微生物的影响.

结论:

开发的方法有效地从小的唾液样本中识别出生物相关的VOC.
整个唾液的独特的VOC特征在很大程度上是由口腔微生物组活动驱动的.
这些发现为开发口腔健康和疾病的非侵入性生物标志物提供了基础.

关键词:

在GC/MS中使用.一个单陷的单陷.这是一种VOC,可挥发性化合物.腺体唾液中的唾液口腔微生物组是口腔的微生物组.这是唾液,唾液.挥发性化合物挥发性的化合物

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