相关实验视频

Updated: Jan 10, 2026

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

非编码RNA作为莱什曼病和查加斯病的新兴生物标志物

Eduardo Ramos Juárez¹, Eduardo Pérez-Campos Mayoral², Laura Pérez-Campos Mayoral²

¹División de Estudios de Posgrado e Investigación, Tecnológico Nacional de Mexico/Instituto Tecnológico de Oaxaca, Oaxaca 68030, Mexico.

Tropical medicine and infectious disease

|November 26, 2025

概括

非编码RNAs (ncRNAs) 在诊断被忽视的热带疾病 (如莱什曼病和查加斯病) 的生物标志物方面表现有前途. 虽然一些ncRNA精确检测莱什曼病感染,但其他ncRNA有助于评估查加斯病的严重程度和心脏并发症.

科学领域:

分子生物学分子生物学
寄生虫学的寄生虫学
生物标志物发现发现

背景情况:

莱什曼病和查加斯病是被忽视的热带疾病,对全球健康产生重大影响.
目前的诊断工具有限,这些疾病的治疗方法有毒.
非编码RNAs (ncRNAs) 由寄生虫释放,并调节宿主-病原体相互作用.

研究的目的:

审查并建议使用ncRNAs作为莱什曼病和查加斯病的诊断标记.
根据2010年至2024年间发表的研究来评估ncRNAs的诊断潜力.
为了区分ncRNAs用于感染诊断与疾病严重程度评估.

主要方法:

来自PubMed,Scopus和谷歌学者 (2010-2024) 的研究的叙事综述.
使用SANRA尺度对研究质量的评估.
对ncRNA表达特征的分析,包括微RNA (miRNA) 和长非编码RNA (lncRNA).

主要成果:

独特的miRNA配置文件 (例如,miR-155-5p) 显示了莱什曼病感染的高诊断准确性 (AUC高达1.0).
血清lncRNAs (MALAT1,NUTM2A-AS1) 显示可能用于莱什曼病诊断,等待验证.
主体miRNAs (例如,miR-21,miR-146a/b) 与慢性查加斯病的心脏参与相关,对心肌病的诊断灵敏度和特异性适度.

结论:

改变的ncRNA表达特征是莱什曼病和查加斯病诊断的有希望的生物标志物.
ncRNAs可以帮助分类物种,治疗监测和评估查加斯病中的心脏并发症.
需要进一步的临床验证,特别是对于莱什曼病中的lncRNAs和早期查加斯感染中的ncRNAs.

关键词:

生物标志物生物标志物查加斯病是查加斯病的一种疾病.细胞外囊泡中的细胞外囊泡.莱什曼尼亚病是一种病.没有编码的RNAs.

更多相关视频

Development of Leishmania Species Strains with Constitutive Expression of eGFP

Development of Leishmania Species Strains with Constitutive Expression of eGFP

Published on: April 21, 2023

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Published on: January 20, 2022

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...