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ESBL-生产的E.E. 黑熊中的大肠杆菌:分子特征和传播风险

Xin Lei¹, Mengjie Che¹, Yuxin Zhou¹

¹Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Veterinary sciences

|November 26, 2025

概括

在被囚禁的黑熊中发现产生扩展谱β-乳糖酶 (ESBL) 的大肠杆菌,显示出高耐药性和水平基因转移能力. 这突出了对熊的潜在威胁,需要监视以防止传播.

科学领域:

微生物学微生物学
兽医医学兽医医学兽医医学
公共卫生公共卫生

背景情况:

产生扩展谱β-乳糖酶 (ESBL) 的大肠杆菌 (ESBL-E. coli) 是一个全球性的健康问题.
关于黑熊中ESBL-E. coli存在的数据有限,包括它们的遗传多样性和水平基因转移 (HGT) 潜力.

研究的目的:

为了表征来自黑熊的ESBL-E.大肠杆菌菌株.
调查这些菌株的抗菌素耐药性概况,序列类型 (ST) 和HGT能力.

主要方法:

多部位序列类型化 (MLST) 用于菌株特征化.
抗微生物敏感性测试.
聚合酶链反应 (PCR) 用于检测β-乳糖抗生素耐药性基因 (ARG).
结合测试用于评估HGT容量.

主要成果:

确定了19种ESBL-E.大肠杆菌菌株 (13.38%).
发现了11种序列类型 (ST),其中包括3种新型ST.
观察到对干amycin/Colistin (KZ),Ceftriaxone (CRO) 和Cefotaxime (CTX) 的高耐药性.
blaCTX-M是最常见的ARG,检测到五种变体.
八种菌株表现出结合性转移,与特定的等离子体和移动遗传元素 (MGE) 共同转移blaCTX-M.

结论:

养黑熊中的ESBL-E. coli菌株具有显著的抗微生物耐药性和HGT潜力.
这些发现表明,对养熊的健康存在潜在威胁.
持续的监测和有针对性的干预措施至关重要,以减轻ESBL-E. coli在这个人群中的传播.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.黑熊是一个黑熊.扩展光谱β-乳糖酶的使用横向基因转移是指水平基因转移.这是β-lactam抗生素的抗生素.

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