相关实验视频

Updated: Jan 10, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

蛋白质与蛋白质相互作用的超高分辨率活细胞映射使用化学遗传分裂报告器和刺激辐射枯竭显微镜.

Stephanie Board1, Arnaud Gautier1,2

  • 1Sorbonne Université, École Normale Supérieure, Chimie Physique et Chimie du Vivant (CPCV), CNRS, Université PSL, 75005, Paris, France.

Chembiochem : a European journal of chemical biology
|November 26, 2025
PubMed
概括

这项研究引入了splitFAST2,一种化学遗传记者,与STED显微镜相结合,以超高分辨率可视化活细胞中的蛋白质-蛋白质相互作用 (PPI). 这一突破使得PPI和在射极限以下的细胞结构能够精确地绘制地图.

关键词:
化学遗传学分裂光记者蛋白质蛋白质相互作用刺激性排放消耗纳米显微镜超高分辨率成像成像技术

更多相关视频

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

11.8K
Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

10.3K

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K