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Updated: Jan 10, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

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蛋白质与蛋白质相互作用的超高分辨率活细胞映射使用化学遗传分裂报告器和刺激辐射枯竭显微镜

Stephanie Board1, Arnaud Gautier1,2

  • 1Sorbonne Université, École Normale Supérieure, Chimie Physique et Chimie du Vivant (CPCV), CNRS, Université PSL, 75005, Paris, France.

Chembiochem : a European journal of chemical biology
|November 26, 2025
PubMed
概括

这项研究引入了splitFAST2,一种化学遗传记者,与STED显微镜相结合,以超高分辨率可视化活细胞中的蛋白质-蛋白质相互作用 (PPI). 这一突破使得PPI和在射极限以下的细胞结构能够精确地绘制地图.

关键词:
化学遗传学分裂光记者蛋白质蛋白质相互作用刺激性排放消耗纳米显微镜超高分辨率成像成像技术

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