迪特尔生物合成的多样化发生在八角珊瑚进化早期
Immo Burkhardt1, Hannah K Bone1, Natalie E Grayson1
1Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093.
概括
深海八角珊瑚是新型类的丰富来源. 它们的烯生物合成途径很早就进化了,这表明这些生物活性化合物在所有八角珊瑚物种的古代起源.
科学领域:
- 海洋生物学 海洋生物学
- 自然产品化学 自然产品化学
- 进化基因组学是进化的基因组学.
背景情况:
- 八角珊瑚是海洋生物活性类的主要生产者.
- 与浅水物种相比,深海八角珊瑚的化学成分在很大程度上仍未被探索.
- 基因组数据现在使得对烯生物合成进化和分布的研究成为可能.
研究的目的:
- 为了描述深海八角珊瑚中的化物化学成分.
- 研究八角珊瑚中烯生物合成途径的进化史.
- 评估深海八角珊瑚作为新生物活性化合物的来源的潜力.
主要方法:
- 收集和化学分析九个深海八角珊瑚标本.
- 对特尔环酶 (TC) 序列的遗传学分析.
- 选择TC基因的功能性特征.
主要成果:
- 发现了多样化的基架和五个广泛分布的二基家族 (XBECK型).
- 遗传学分析显示TC基因与深层分类学起源相对应,而不仅仅是生物化学功能.
- 识别了五个单基细胞系的异功能酶,它们在各个种类中产生XBECK类型的双类前体.
结论:
- 类生物合成是八角珊瑚的古老特征,存在于它们的共同祖先中.
- 深海八角珊瑚具有发现新生物活性类的巨大潜力.
- 这些发现促使进一步研究这些途径在早期的类动物和以后的进化起源.
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