相关实验视频

Updated: Jan 10, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

人类骨肌肉中的表观遗传衰老特征和年龄预测

Soo-Bin Yang¹, Jeong Min Lee², Moon-Young Kim³

¹Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea.

|November 26, 2025

概括

这项研究在韩国成年人中使用DNA甲基化开发了骨肌肉表观遗传钟. 这些新型模型可以从死后的肌肉组织中准确地预测时间的年龄,帮助法医科学和衰老研究.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
老年学是一门学科.

背景情况:

衰老通过分子和细胞变化损害了骨肌肉功能.
基因甲基化是衰老的关键表观遗传调节剂,但它在骨肌肉中的作用,特别是在亚洲人群和死后样本中,是研究不足的.

研究的目的:

分析韩国人死后骨肌肉中的DNA甲基化概况.
开发和验证表观遗传钟用于骨肌肉的年龄预测.
为了识别肌肉组织中与年龄相关的甲基化特征.

主要方法:

使用Infinium EPIC阵列对103个胸部主要肌肉尸检样本的DNA甲基化概况进行分析.
使用下一代测序 (NGS) 和单基扩展 (SBE) 进行有针对性的验证和年龄预测建模.
基于已识别的与年龄相关的CpG标记器开发机器学习模型.

主要成果:

鉴定了20种与年龄相关的CpG标记物,这些标记物与参与肌肉结构,新陈代谢和应激反应的基因有关.
机器学习模型展示了高年龄预测精度 (NGS的MAE为5.537年,SBE为3.797年) 和与时间年龄的强烈相关性.
在亚洲人群中使用尸体组织成功建立了骨肌肉表观遗传钟.

结论:

新的骨肌肉表观遗传钟是为亚洲人群开发的,使用尸体样本.
20-CpG标志物模型为法医应用和定制为人群的表观遗传特征提供了一个强大的框架.
识别的甲基化特征为研究和临床干预提供了关于肌肉衰老途径和潜在生物标志物的见解.

关键词:

通过DNA甲基化.年龄的年龄年龄的年龄.下一代测序的下一代测序.单个基础扩展的单个基础扩展.这是骨肌肉的骨架肌肉.

更多相关视频

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies

Published on: December 1, 2023

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Published on: October 26, 2017

相关概念视频

The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...