基于网络药理学,代谢学和转录学的多omics分析,对治疗乙型肝炎的HuaganJiedu的机制研究
Hongxuan Tong1, Jiale Zhang1, Lijie Jiang1
1Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
概括
华杰杜 (HGJDD) 有效地降低了乙型肝炎病毒 (HBV) 标记物. 多omics分析揭示了FXR/RXRα-SHP1-HNF1α轴作为HBV治疗中HGJDD的关键治疗机制.
科学领域:
- 综合医学是一个整体的医学.
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 传统中医 (TCM) 提供草药药物,如HuaganJiedu开 (HGJDD) 作为潜在的辅助乙型肝炎病毒 (HBV) 治疗.
- 在改善HBV患者的临床结果方面,HGJDD已经显示出有效性.
研究的目的:
- 使用多omics方法阐明HGJDD在HBV治疗中的治疗机制.
- 研究HBV治疗中HGJDD向的分子通路.
主要方法:
- 液态染色学-质谱学 (LC-MS) 确定了HGJDD的组成部分.
- 试验室 (HepG2.2.15细胞) 和体内 (HBV-TG小鼠) 研究评估了HGJDD的抗病毒作用.
- 多omics分析 (网络药理学,代谢学,转录学) 确定了关键机制,包括FXR/RXRα-SHP1-HNF1α轴.
主要成果:
- HGJDD显著降低了乙型肝炎表面抗原 (HBsAg),乙型肝炎e抗原 (HBeAg) 和HBV DNA水平.
- 多omics分析确定了FXR/RXRα-SHP1-HNF1α轴作为主要的治疗点.
- 分子对接和实验验证证证实了HGJDD组件与确定轴之间的相互作用.
结论:
- FXR/RXRα-SHP1-HNF1α轴是HGJDD抗HBV活性的主要机制.
- HGJDD是HBV的一种有前途的治疗药物,通过调节这一关键分子通路而起作用.
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