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功能化的激光诱导石墨烯启用了超敏感的电免疫试验,用于快速检测乙型肝炎病毒

Akkapol Suea-Ngam¹, Tidawan Ngamyoo¹, Orawon Chailapakul¹

¹Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.

Bioelectrochemistry (Amsterdam, Netherlands)

|November 26, 2025

概括

一个新的电化学生物传感器使用激光诱导的石墨烯检测乙型肝炎表面抗原 (HBsAg). 这种快速,敏感的诊断工具提供了切实可行的早期查乙型肝炎病毒 (HBV) 感染,特别是在资源有限的环境中.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
材料科学材料科学材料科学

背景情况:

乙型肝炎病毒 (HBV) 感染是一个重大的全球健康问题.
目前对HBV的诊断需要提高速度,灵敏度和成本效益.
需要先进的诊断工具来有效管理疾病和公共卫生监测.

研究的目的:

开发一种高性能电化学免疫传感器,用于超标检测乙型肝炎表面抗原 (HBsAg).
利用激光诱导石墨烯 (LIG) 技术克服传统HBV诊断的局限性.
为早期乙型肝炎查创建一个灵敏,快速和具有成本效益的诊断平台.

主要方法:

使用二氧化碳激光工艺在聚胺上制造一个多孔的,三电极的石墨烯传感器.
通过电化学氧化对石墨烯传感器的功能化,使得共价抗体固定.
使用固态阻碍原理和方波电压测量检测HBsAg.

主要成果:

免疫传感器显示,缓冲剂的超低检测极限为0.04 pg mL-1和人血清中的0.18 pg mL-1.
从1 × 10−4到1 × 101 ng mL−1.1.1实现了广泛的线性检测范围.
对病毒干扰剂和30天内的稳定性表现出极好的选择性.

结论:

开发的电化学免疫传感器提供了实验室级别的灵敏度,同时具有护理点的实用性.
这种生物传感器是早期有效查乙型肝炎病毒感染的有希望的工具.
该技术有可能改善HBV诊断,特别是在资源有限的环境中.

关键词:

生物传感器是一种生物传感器.电化学检测电化学检测乙型肝炎 B 型肝炎激光诱导的石墨烯是一种激光.

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