Pseudomonas marginalis 的适应性反应对改变了大气的包装条件
Muhammad Asim1, Huihui Xu1, Yanyin Guo1
1College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255049, People's Republic of China.
International journal of food microbiology
|November 26, 2025
概括
修改大气包装 (MAP) 允许Pseudomonas marginalis通过改变细菌应激和代谢途径来适应并引起番茄软腐烂. 快速转移到受控大气层可以减轻这些收获后损失.
科学领域:
- 食品微生物学 食品微生物学
- 植物病理学 植物病理学
- 微生物生理学 微生物生理学
背景情况:
- 收获后的细菌感染显著影响水果和蔬菜的质量和安全.
- 病原体适应储存条件加剧了腐败.
- 马格里纳斯虫 (Pseudomonas marginalis) 导致番茄软腐烂,这是收获后的一个主要问题.
研究的目的:
- 在修改大气包装 (MAP) 条件下调查Pseudomonas marginalis的生存和软腐烂进展.
- 通过多omics方法阐明P. marginalis对MAP的适应性反应.
- 为了确定由MAP气体调节的关键细菌通路.
主要方法:
- 使用了形态分析和多组学 (转录组学和代谢组学).
- 在不同的大气条件下评估了细菌的生长和活力.
- 用RT-qPCR,转录组学和代谢组学分析了基因表达和代谢物概况.
主要成果:
- MAP支持P. marginalis与空气相似的生长,而快速转移到受控大气抑制了生长,导致了膜破坏.
- MAP增强了细菌的适应性反应,保持了适度的生存能力.
- MAP调节了细菌的两组分系统 (TCS) 和ATP结合盒 (ABC) 输送通路,改变了铁的稳态,并影响了氨基酸和糖的新陈代谢.
结论:
- 通过调节关键的压力和代谢途径,MAP促进了P. marginalis的适应和软腐烂的发展.
- 快速转移到微空气控制大气或活跃的氧气清理可以减轻P. marginalis诱导的收获后损失.
- 这些发现为优化MAP策略的基础,以控制产品中的细菌腐烂.
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