相关实验视频

Updated: Aug 12, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

假名V通过抑制PPARGC1A的m6A修饰来减轻ox-LDL诱导的巨细胞M1两极化

Tao Li¹, Qi Pang², Jun Pan³

¹Department of Cardiology, Lanzhou Petrochemical General Hospital (The Fourth Affiliated Hospital of Gansu University of Chinese Medicine), Lanzhou, 730060, China.

Experimental cell research

|November 26, 2025

概括

氧化LDL会触发N6-甲基氨酸 (m⁶A) RNA的变化,促进M1巨细胞的两极分化和动脉样硬化. 海洋天然产品Pseudane V可以逆转这些m⁶A修改,提供潜在的动脉样硬化预防.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
心血管研究研究心血管研究

背景情况:

动脉样硬化病原包括由氧化低密度脂蛋白 (ox-LDL) 驱动的巨细胞功能障碍.
精确的分子机制,包括RNA甲基化,控制牛-LDL诱导的巨细胞极化尚未完全理解.
向巨细胞两极分化为动脉样硬化的治疗策略.

研究的目的:

阐明N6-甲基亚丁素 (m⁶A) RNA甲基化在ox-LDL诱导的M1巨细胞极化中的作用.
为了识别牛-LDL-m6的下游目标.巨细胞中的一种途径.
发现能够逆转牛-LDL驱动的巨细胞极化干预动脉样硬化的海洋天然产品.

主要方法:

人类单细胞THP-1细胞被分化并用ox-LDL处理.
通过流细胞计和qRT-PCR评估了巨细胞极化 (M1/M2).
全球m⁶A水平使用点滴分析;MeRIP-seq和RNA-seq确定了目标基因.
对海洋天然产品进行了高通量选.

主要成果:

氧-LDL诱导了M1巨分化,与增加的全球RNAm6A修饰有关.
一个被抑制的PPARGC1A表达以YTHDF2依赖的方式,促进M1极化.
海洋天然产品Pseudane V通过使PPARGC1A m⁶A修改正常化,逆转了ox-LDL诱导的M1极化,并保护了内皮细胞.

结论:

氧-LDL通过增加PPARGC1A的m6的修饰来促进动脉样硬化,抑制其表达并驱动M1巨细胞两极分化.
伪丹V有效地抵消ox-LDL诱导的m⁶A变化,抑制M1极化.
假名V证明了作为预防动脉样硬化的治疗剂的潜力.

关键词:

动脉样硬化是一种动脉样硬化.巨细胞是一个巨细胞.这就是PPARGC1A.伪名人V 伪名人V m(6) A6) 一个

更多相关视频

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...