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肝细胞癌中的EZH2表达及其与昼夜节律相关基因的关系

Xingyue Wang¹, Miaolong He², Limian Zhou¹

¹Department of Nuclear Medicine, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, Jiangsu, China.

Scientific reports

|November 26, 2025

概括

增强性同源2 (EZH2) 在肝细胞癌 (HCC) 中过度表达,与预后不佳相关,并影响昼夜节律基因. 向EZH2可能为肝癌提供新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

肝细胞癌 (HCC) 由于发病率不断增加和死亡率高,对全球健康构成了重大挑战.
病毒性肝炎感染和肝硬化是导致HCC发展和进展的主要危险因素.

研究的目的:

调查增强器的作用胃同源2 (EZH2) 在肝细胞癌 (HCC) 的进展和预后.
探索HCC.中EZH2表达和昼夜节律相关基因之间的关联.
评估EZH2作为潜在的预后生物标志物和HCC的治疗点.

主要方法:

对HCC数据集 (TCGA,GEO) 的生物信息分析,以确定差异表达的基因和核心候选者.
蛋白与蛋白相互作用网络,LASSO和Cox回归分析用于基因查.
功能丰富,免疫透分析 (GSVA,ssGSEA) 和甲基化分析 (MethSurv).
实验验证使用西部抹杀,qRT-PCR,siRNA敲除和功能测试.

主要成果:

发现EZH2在HCC组织中显著过度表达,与晚期瘤阶段和患者生存率差相关.
EZH2表达与Th2细胞和树突细胞的透增加,以及促进物超甲基化有关.
EZH2的淘汰抑制了HCC细胞的增殖,诱导了DNA损伤,并降低了昼夜基因CLOCK和CRY1.
一个结合EZH2的预后名录显示出对HCC结果的强大预测准确性.

结论:

EZH2在调节HCC进展方面发挥着至关重要的作用,并与昼夜节律障碍有关.
EZH2作为一种潜在的预后生物标志物和肝细胞癌的有前途的治疗点.
表观遗传修饰,特别是促进剂高甲基化,影响EZH2表达和HCC存活率.

关键词:

生物标志物生物标志物循环节律是循环节律的节奏.在 EZH2 中使用.肝细胞癌是肝细胞癌.预测预测预测预测

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