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雷米马佐拉姆诱导的麻醉不会显著影响小鼠的昼夜节律

Yasuhito Suzuki¹, Ryo Imai², Yoshitaka Aoki¹

¹Department of Anesthesiology and Intensive Care, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka, 431-3192, Japan.

Scientific reports

|November 26, 2025

概括

雷米马佐拉姆 (RMZ) 是一种超短效的二,对小鼠的昼夜节律和SCN中的时钟基因表达产生最小的影响. 这表明RMZ可能会减少麻醉相关的昼夜干扰并发症.

科学领域:

神经科学是一个神经科学.
麻醉学麻醉学
时间生物学时间生物学

背景情况:

哺乳动物的昼夜节律由上神核 (SCN) 中的时钟基因调节.
吸入性麻醉会扰乱昼夜节律,但静脉内二二胺 (BDZ) 诱导的麻醉的影响不太清楚.
雷米马佐拉姆 (RMZ) 是一种超短作用的BDZ,在临床麻醉中越来越多地使用.

研究的目的:

研究雷米马佐拉姆 (RMZ) 对SCN中昼夜节律和时钟基因表达的影响.
评估在小鼠中RMZ给药后的行为休息活动模式和时钟基因mRNA水平.

主要方法:

雄性C57BL/6J小鼠在持续的黑暗条件下接受连续或玻尿酸RMZ注射,或盐水控制.
通过测量相位变化和活动分布来评估行为昼夜节律.
使用定量实时PCR和PER2::LUC生物发光测试来分析SCN中的时钟基因表达.

主要成果:

用RMZ给药并没有导致行为昼夜节律的显著相位变化.
在各组之间没有观察到活动分布或不活动发作的显著差异.
虽然RMZ降低了Per2,Bmal1,Cry1和Npas2的mRNA水平,并在SCN中增加了Egr1,但它没有改变Per2昼夜振荡的周期.

结论:

在经过测试的实验条件下,雷米马佐拉姆对昼夜节律和时钟基因表达的干扰最小.
RMZ显示了与全身麻醉诱导的昼夜节律扰乱相关的并发症的缓解潜力.
需要进一步的研究,以充分阐明RMZ对昼夜生理学的临床影响.

关键词:

二甲胺类药物二胺类药物循环节律循环节律是指循环节律.外科手术期间的并发症.雷米马佐拉姆 (Remimazolam) 是一种药物.

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