在反动物的早期胎盘发育期间,对保存的细胞类型特异机制的跨物种识别
Morgan L Leavitt1, Amanda L Patterson2,3, Morgan D Wagle1
1Department of Animal Sciences, Washington State University, Pullman, WA, USA.
Scientific reports
|November 26, 2025
概括
了解牛和羊的早期胎盘发育是预防流产的关键. 这项研究揭示了表皮细胞到介质细胞的过渡 (EMT) 在早期的反动物胎盘期间在热囊细胞中至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 进行比较基因组学.
背景情况:
- 在反动物,特别是牛群中,怀孕失败通常源于怀孕20天后的胎盘发育失败.
- 对胎盘正常发育的详细,特定于细胞类型的理解对于确定流产的原因至关重要.
研究的目的:
- 在单细胞分辨率下研究和比较羊和牛的早期胎盘发育.
- 在早期胎盘期间识别保存和特定物种的细胞过程和细胞群.
主要方法:
- 集成的单细胞RNA测序 (scRNA-seq) 数据来自绵羊和牛的胎盘组织.
- 生物信息分析包括细胞聚类,伪时代分析和细胞-细胞信号通路调查.
- 基因本体学丰富分析用于比较研究.
主要成果:
- 在两种物种中确定了19个不同的细胞种群,具有保存的介质细胞,上皮细胞和热囊细胞形状.
- 发现了两种牛特异性热囊细胞群,表达IFNT2和CSH2/PAG17.
- 观测到上细胞转移到介质细胞转变 (EMT) 基因的动态表达,得到了伪时代和信号分析的支持.
结论:
- 表皮细胞到介质细胞的过渡 (EMT) 似乎是早期反动物热囊细胞发育中的关键和保存过程.
- 研究结果表明,在反动物和人类外性热囊细胞之间存在着保存的EMT机制.
- 这项研究为早期胎盘发育提供了基础细胞类型层面的洞察力,这对于解决牲畜妊娠流产至关重要.
相关概念视频
01:56Gastrulation
65.8K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
65.8K
01:51Determination
20.7K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.7K