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编辑基因-12基因会影响Helicoverpa zea对Bt毒素Cry1Ac的反应

Chan C Heu¹, Kyle M Benowitz², Luciano M Matzkin³

¹USDA ARS, U.S. Arid Land Agricultural Research Center, Maricopa, AZ, 85138, USA.

Scientific reports

|November 26, 2025

概括

昆虫对Bacillus thuringiensis (Bt) 作物的耐药性是一个日益严重的问题. 这项研究表明,kinesin-12基因对于Bt作物对害虫Helicoverpa zea的有效性至关重要.

科学领域:

农业昆虫学农业昆虫学
分子遗传学分子遗传学
虫害管理虫害管理虫害管理

背景情况:

基因工程培养的Bacillus thuringiensis (Bt) 作物对于控制昆虫害虫至关重要.
昆虫对Bt蛋白质的耐药性的演变,包括Bt棉花中的Cry1Ac,显著降低了作物的有效性.
赫利科瓦尔帕泽亚 (Helicoverpa zea) 对Cry1Ac具有抗性,在美国构成重大经济威胁.

研究的目的:

为了研究基因素-12基因在Helicoverpa zea对Cry1Ac.抗性的作用.
为了验证之前一项全基因组关联研究的发现,该研究将基因素-12突变与Cry1Ac耐药性联系起来.

主要方法:

利用CRISPR/Cas9基因编辑技术修改了Helicoverpa zea.中的kinesin-12基因.
在一个敏感菌株中敲除了kinesin-12基因,以评估其对Cry1Ac耐药性的影响.
修复了一种耐药菌株的kinesin-12基因中已知的无意义突变,以评估易感性变化.

主要成果:

在敏感的H. zea中淘汰kinesin-12基因增加了4.0倍的Cry1Ac抗性.
修复耐药H. zea中的kinesin-12无意义突变,使得对Cry1Ac的敏感性增加了3.8倍.
基因编辑结果证实了基因素-12在Cry1Ac毒性中的重要作用.

结论:

基因-12基因是Cry1Ac对Helicoverpa zea的作用模式的一个关键因素.
了解Bt耐药性的遗传基础对于开发可持续的害虫管理策略至关重要.
这项研究为农业中管理Bt耐药昆虫种群提供了基础.

关键词:

细菌 thuringiensis 这种疾病.基因编辑CRISPR/Cas9基因编辑玉米耳玉米耳玉米耳昆虫杀虫剂耐药性昆虫杀虫剂耐药性这种类型的激素是kinesin.转基因Bt作物转基因Bt作物

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