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通过PPARγ信号通路促进山羊肌内脂肪中的脂质重构

Yuhan Dai¹, Yuling Yang¹, Haiyang Li¹

¹Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Key Laboratory of Sichuan Province, Southwest Minzu University, Chengdu 610041, China.

|November 27, 2025

概括

皮利平5 (PLIN5) 基因通过影响前脂肪细胞增殖和脂质代谢来调节山羊肌内脂肪沉积. 它激活PPARγ和PI3K-AKT通路,影响肉质.

科学领域:

动物科学动物科学
分子生物学分子生物学
生物化学生物化学

背景情况:

肌肉内脂肪 (IMF) 含量对山羊肉质量至关重要,受到前脂肪细胞行为的影响.
脂质滴蛋白Perilipin 5 (PLIN5) 在山羊IMF沉积中的作用在很大程度上是未知的.

研究的目的:

阐明PLIN5基因在调节山羊肌肉内脂肪沉积中的功能和机制.
研究参与PLIN5介导脂质代谢的信号通路.

主要方法:

在山羊预脂细胞中过度表达和抑制PLIN5.
对细胞增殖,细胞亡和脂质沉积的分析.
非向性脂组学测序和KEGG通路丰富分析.
使用PI3K-AKT和PPARγ抑制剂的救援实验.

主要成果:

过度表达PLIN5促进了亡,减少了增殖,并在山羊前脂肪细胞中增加了脂质沉积.
在PLIN5上调的过氧体增殖器激活受体玛 (PPARγ) 和脂质发生基因.
脂管学发现了差异表达的脂质,主要涉及PI3K-AKT通路.
特定的 lysophosphatidylinositols (LPIs) 被上调并与PPARγ激活相关.

结论:

PLIN5通过PPARγ和PI3K-AKT信号通路调节山羊肌肉内脂肪沉积.
了解PLIN5的作用为通过IMF调制改善山羊肉质量提供了洞察力.

关键词:

国际货币基金组织国际货币基金组织国际货币基金组织通过PI3K-AKT途径.在PLIN5中,有5个字母.在PPARγ中,PPARγ是PPARγ 肌肉内脂肪细胞是什么

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