模仿miR-71的T7合成双链RNA诱导白RNAi并增加真菌的有效性
Chenchen Zhao1,2, Hang Lu1, Ruotian Cheng1
1Henan International Laboratory for Green Pest Control, Henan Engineering Laboratory of Pest Biological Control, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Biomolecules
|November 27, 2025
概括
微RNA-71 (miR-71) 通过增加真菌诱导的死亡率来增强生物虫控制. 这项研究证实了它的有效性,并通过确定由miR-71模仿分子调节的关键防御基因来探索它的机制.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 众所周知,microRNA-71 (miR-71) 有助于增强对白的生物控制剂.
- miR-71增强虫控制的确切机制尚不清楚.
研究的目的:
- 研究miR-71在白基因表达和感染死亡率中的功能作用.
- 开发和比较一种具有成本效益的方法来合成miR-71模仿物,使用T7RNA聚合酶与商业miR-71 agomir.
主要方法:
- 使用商业 miR-71 agomir 和 T7 合成的 miR-71 模仿剂进行了比较生物测试.
- 进行了转录组和蛋白组分析,以评估基因表达变化.
- 使用RT-qPCR来确认基因表达调制.
- 通过dSRNA介导沉默的RNA干扰 (RNAi) 用于验证基因功能.
主要成果:
- 商业 miR-71 agomir 和 T7 合成的 miR-71 都模仿了明显增加的真菌诱导的白死亡率,具有可比的生物活性.
- 转录基因和蛋白质基因分析显示,miR-71调节了与防御相关的基因的表达,包括赫萨梅林-1,神经灵-4和可能的酶-10.
- 沉默这些防御基因使白更容易受到真菌感染,证实了它们在白防御中的作用.
结论:
- 在体外转录的miRNA模拟器是研究microRNA-mRNA相互作用的有效和经济的工具.
- 这项研究为使用微生物媒介表达小RNA模仿物来改进白生物控制策略提供了基础.
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