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多组学分析揭示了在Yarrowia lipolytica中的1 - 醇诱导的五甲酸生物合成

Jiahe Cong¹, Xin Hu¹, Dongsheng Lu¹

¹Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

|November 27, 2025

概括

补充Yarrowia lipolytica的1-propanol显著增加了甲酸 (C15:0) 的产量. 这项研究确定了1-醇作为一种有效的前体,通过微生物发酵增强了C15:0产量.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
代谢工程是代谢工程.

背景情况:

五甲酸 (C15:0) 是一种奇链脂肪酸 (OCFA),具有健康益处,主要通过微生物发酵产生.
优化C15:0生产对于其在健康和营养领域的更广泛应用至关重要.

研究的目的:

调查不同酒精作为前体的有效性,以增强Yarrowia lipolytica中的C15:0产量.
为了确定最有效的酒精前体的最佳度.
通过转录组和代谢组分析,阐明基于增强C15:0合成的分子机制.

主要方法:

选各种酒精对它们对Yarrowia lipolyticaCICC1778.0产生的C15:0产生的影响
优化1-醇度以获得最大的C15:0产量.
综合转录组和代谢组分析以确定参与生产途径的关键基因和代谢物.

主要成果:

鉴定出1-醇是最有效的酒精前体,可显著增加C15:0产量.
用0.5%的1-propanol补充剂导致C15:0产量 (76.68 mg/L) 增加794.7%,而不会影响总脂质含量.
综合的奥米克数据揭示了参与propionyl-CoA代谢和分支链氨基酸降解的基因和代谢物的上调.

结论:

补充0.5%的1-propanol是一种高度有效的策略,用于增强Yarrowia lipolytica中的C15:0产量.
1-propanol通过脱氧化和分支链氨基酸降解来促进propionyl-CoA池的扩张来增强C15:0合成.
这项研究为Yarrowia lipolytica中OCFA产生的代谢调节提供了宝贵的见解.

关键词:

1-烯醇的使用方法雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.代谢生物组的代谢生物组甲酸是甲酸的一种.翻译学翻译学翻译学翻译学

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