假定β-乳糖酶是否构成潜在的未来威胁?
Patrik Mlynarcik1, Veronika Zdarska1, Milan Kolar1
1Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 77515 Olomouc, Czech Republic.
Antibiotics (Basel, Switzerland)
|November 27, 2025
概括
本研究通过分析来自不同来源的酶候选物来确定潜在的β-乳糖酶威胁. 一个新的框架优先考虑基于遗传特征的酶,帮助全球抗菌素耐药性监测.
科学领域:
- 基因组学和生物信息学
- 微生物学 微生物学
- 公共卫生 公共卫生
背景情况:
- 抗微生物耐药性 (AMR) 是一个重大的全球健康挑战,β-乳糖酶赋予了对关键β-乳糖抗生素的耐药性.
- 之前的一项调查确定了2340个潜在的β-乳糖酶,促使对129个优先候选者的集中分析.
研究的目的:
- 开发和验证一个框架,用于早期检测和优先考虑新的β-lactamase候选者.
- 评估这些耐药性基因在不同细菌储存库中的水平基因转移和流动性的潜力.
主要方法:
- 对来自环境,动物和人类来源的129个优先考虑的β-乳糖酶候选物的分析.
- 催化残留的基因中心评估和基因组背景的评估,使用近位移动性指数 (IPM).
- 遗传学分析和基于人工智能的基质预测,以获得探索性见解.
主要成果:
- 候选人代表所有Ambler类 (A-D) 具有保存的催化动机,表明潜在的活动.
- 9%的候选基因位点显示高IPM,表明基因组背景有利于水平基因转移,通常靠近移动元素.
- 在多个属和大陆发现了相同的A类酶,经常与调动蛋白相关联.
结论:
- 提出了一个可重复的,有偏见的框架,以根据动机完整性和移动性背景来优先考虑β-乳糖酶候选者.
- 该框架支持全球监测倡议 (GLASS/EARS-Net),并整合了"一个健康"的方法.
- 实验验证对于确认优先候选者的功能,移动性和临床相关性至关重要.
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