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作为潜在炎症标记物的β-Casomorphin-7:β-Casomorphin-7如何诱导HUVEC/TERT2细胞系中的内皮功能障碍

Judit Rita Homoki¹, Emese Szilágyi-Tolnai¹, Ildikó Kovács-Forgács¹

¹Center for Complex Systems and Microbiome Innovations, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, H-4032 Debrecen, Hungary.

|November 27, 2025

概括

β-casomorphin-7 (BCM-7) 可能通过增加氧化应激和炎症来恶化内皮功能障碍. 在A1β-casein中发现的这种会加剧这些效应,特别是与脂聚糖 (LPS) 结合时.

科学领域:

分子生物学分子生物学
心血管研究研究心血管研究
免疫学免疫学免疫学

背景情况:

内皮功能障碍是心血管疾病发展的关键因素.
疑似从A1β-casein中获得的β-casomorphin-7 (BCM-7),可能会导致内皮功能障碍.
对于BCM-7对内皮细胞的直接影响仍然在很大程度上未被研究.

研究的目的:

调查BCM-7是否诱导内皮细胞功能障碍.
确定BCM-7是否会增加炎症性细胞因子和活性氧物种 (ROS).
评估BCM-7和脂多糖 (LPS) 对内皮细胞的联合作用.

主要方法:

人类静脉内皮细胞 (HUVECs/TERT2) 用BCM-7 (5μg/mL) 和LPS (100 ng/mL) 进行治疗.
评估了细胞活力,细胞亡,亡和细胞内ROS.
使用定量实时PCR进行量化的促炎细胞因子和调节炎症的酶.

主要成果:

BCM-7显著增加了细胞内ROS的产生和IL-6和IL-8的表达.
当BCM-7与LPS结合使用时,这些效应被放大了.
BCM-7改变了关键酶的表达:减少了COX-1,增加了COX-2,LOX-5,NOS3和PTGDS.

结论:

通过增强氧化应激和炎症反应,BCM-7有助于内皮功能障碍.
LPS的存在使BCM-7对内皮细胞的有害影响变得更大.
研究结果强调BCM-7是心血管疾病发病的潜在因素.

关键词:

在HUVECEC中使用.细胞内皮细胞的恒常性.内皮质功能障碍内皮质功能障碍这是一种炎症炎症炎症炎症.血管生理学血管生理学公司的可行性,可行性.贝塔-卡索莫尔芬-7-7的使用情况

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