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在之前流产的妇女的植入窗口期间改变了子宫内膜内存T细胞配置文件

Dimitar Parvanov¹, Rumiana Ganeva¹, Margarita Ruseva¹

¹Department of Research, Nadezhda Women's Health Hospital, 1330 Sofia, Bulgaria.

|November 27, 2025

概括

有流产史的女性在植入窗口期间改变了子宫内膜记忆T细胞,显示了更多的中央记忆T细胞和更少的效应记忆T细胞,可能会影响未来的怀孕.

科学领域:

免疫学免疫学免疫学
生殖生物学生殖生物学
细胞生物学细胞生物学

背景情况:

记忆T细胞子集 (中心,效应,组织居民,终端分化) 在局部免疫反应中起着至关重要的作用.
子宫内膜免疫细胞组成,特别是T细胞,对于成功的胚胎植入至关重要.
以前的流产可能会在植入窗口期间改变子宫内膜的局部免疫环境.

研究的目的:

描述和比较中部 (TCM),效应器 (TEM),组织居民 (TRM) 和终端分化 (TEMRA) 记忆T细胞子集在子宫内膜中.
在植入窗口 (WOI) 期间,在有或没有流产史的女性之间调查这些记忆T细胞子集的差异.
评估改变记忆T细胞网络对植入准备的组织和潜在影响.

主要方法:

从在中期的乳房阶段 (P+5) 收集的子宫内膜样本中分离了流体淋巴细胞.
采用多色流细胞计量来量化CD4+和CD8+TCM,TEM,TRM和TEMRA的T细胞子集.
统计分析,包括相关性,主要组件分析 (PCA) 和后勤回归,用于比较群组并分析网络组织.

主要成果:

有流产史的女性在总和CD8+淋巴细胞中显示出显著更高比例的中央记忆T细胞 (TCM).
与对照组相比,这些女性也表现出较低的CD8+效应记忆T细胞 (TEM) 和较高的CD4+TEM比例.
组织内存T细胞 (TRM) 呈现增长趋势,TCM/TEM比率成为关键的歧视性参数.

结论:

在WOI期间,子宫内膜内存T细胞的组成在有流产史的女性中发生了变化,有利于较少差异化的表型.
观察到的转向TCM和远离TEM的转变表明本地免疫记忆网络的潜在重塑.
这些免疫变化可能会影响子宫内膜受体性和在随后的月经周期中为植入做好准备.

关键词:

中央记忆T细胞 (TCM)效应器记忆T细胞 (TEM) 的作用人类子宫内膜记忆T细胞记忆T细胞流产流产流产流产生殖免疫学生殖免疫学终端分化的效应器内存T细胞 (TEMRA)组织内存T细胞 (TRM)植入的植入窗口的时间.

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